基于Cu^(2+)-2,6-双(2-苯并咪唑)吡啶配合物的巯嘌呤荧光分析法  

Spectrofluorometry of Mercaptopurine Based on Cu^(2+)-2,6-bis(2-benzimidazyl)pyridine Complex

作　　者：杨亚梅[1,2] 赵希娟[1,2] 甄淑君[1,2] 李原芳[1,2] 

机构地区：[1]发光与实时分析教育部重点实验室,重庆400715 [2]西南大学化学化工学院,重庆400715

出　　处：《西南大学学报（自然科学版）》2012年第9期59-63,共5页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(21175109)

摘　　要：基于配体竞争反应提出了抗癌药物巯嘌呤的荧光分析法.研究发现,在pH=6.8的HEPES缓冲中,Cu2+能与荧光小分子2,6-双(2-苯并咪唑)吡啶(Bbimp)形成非荧光配合物Cu2+-Bbimp,但当抗癌药物巯嘌呤(MP)存在时,由于MP中的巯基(—SH)与Cu2+发生强烈作用而发生配体竞争,游离出的Bbimp的荧光与MP具有函数关系.据此建立了MP的荧光分析方法,线性范围为4.0×10-6～7.0×10-5 mol/L,检测限为3.0×10-7 mol/L.该方法成功用于人体尿样中MP的分析,回收率在96.7%～107.3%之间,相对标准偏差(RSD)小于1.97%.测定结果与药典标准方法一致.The analysis and quality control of anti-cancer drugs is a new field for modern pharmaceutical analysis.Taking mercaptopurine（MP） as an example,we proposed a spectrofluorometric analysis for anti-cancer drug based on the competitive reaction of ligands.In an HEPES buffer solution（pH=6.8）,Cu2＋ was found to interact with 2,6-bis（2-benzimidazyl）pyridine（Bbimp）,forming a non-fluorescent Cu2＋-Bbimp complex.In the presence of the anti-cancer drug mercaptopurine（MP）,Bbimp was released owing to ligand competition,in which Cu2＋ can firmly combine with —SH of MP.Interestingly,the fluorescence of released Bbimp showed a linear relationship with MP concentrations.Therefore a spectrofluorometric method for MP detection was developed.The linear range of this method was 4.0×10-6-7.0×10-5 mol/L,with a detection limit（3σ/k） of 3.0×10-7 mol/L.This method has been applied for the determination of MP in human urine samples with a recovery of 96.7%-107.3% and a relative standard deviation（RSD） of less than 1.97%.The results were consistent with those obtained with the standard method in China Pharmacopoeia.

关 键 词：荧光分析法 2 6-双(2-苯并咪唑)吡啶 巯嘌呤 铜离子 

分 类 号：O657.3[理学—分析化学]