广西烤烟棒孢霉叶斑病病原分子鉴定及其生物学特性补充  

Molecular identification and a supplement to the biological characteristics of the pathogen causing flue-cured tobacco corynespora leaf spot in Guangxi

作　　者：谭海文[1] 卢燕回 王雅[1] 陈振东[1] 袁高庆[1] 林纬[1] 黎起秦[1] 

机构地区：[1]广西大学农学院,南宁530004 [2]广西区烟草公司,南宁530023

出　　处：《植物保护》2012年第5期35-39,50,共6页Plant Protection

基　　金：广西烟草有害生物调查研究项目

摘　　要：本文对广西烤烟棒孢霉叶斑病的病原进行鉴定,并对其生物学特性进行初步研究。根据该病原菌的形态特征、致病性及rDNA-ITS序列测定,将引起广西烤烟棒孢霉叶斑病的病原菌鉴定为山扁豆生棒孢(Corynespora cas-siicola)。生物学特性测定结果表明:27.5℃、培养初始pH为6、以麦芽糖作为C源、硝酸钾作为N源及完全光照等条件最适合其菌丝生长;30℃、以乳糖作为C源、硝酸钾作为N源及完全黑暗等条件最有利于该菌分生孢子形成;27.5℃、湿度为100%+水膜、12h光暗交替等条件最有利于分生孢子萌发;分生孢子的致死温度为52℃。这是该病在广西的首次报道。The identification of the pathogen causing flue-cured tobacco corynespora leaf spot in Guangxi and its biological characteristics were preliminarily studied.The pathogen of flue-cured tobacco corynespora leaf spot was identified as C.cassiicola based on its morphology,pathogenicity and the analysis of sequences of ribosomal rDNA-ITS.The optimum temperature,pH value,carbon source,nitrogen source and light treatment for the mycelial growth of C.cassiicola were 27.5 ℃,6,maltose,potassium nitrate and full light,respectively.For the sporulation,the optimum temperature was 30 ℃,and the best carbon/ nitrogen source was lactose/ potassium nitrate,and full darkness was the most suitable light treatment.For conidial germination,the most favorite temperature,relative humidity and light treatment were 27.5 ℃,100% and 12 h alternate light,respectively.The lethal temperature for the spores was 52 ℃.It is the first report of the tobacco disease in Guangxi,China.

关 键 词：烤烟棒孢霉叶斑病 山扁豆生棒孢 鉴定 生物学特性 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]