对斜纹夜蛾高效绿僵菌的筛选  

Screening of high virulence strains of Metarhizium anisopliae against Spodoptera litura

作　　者：马丽娟[1] 滕忠才[2] 臧欢[1] 刘廷辉[1] 李瑞军[1] 郭巍[1] 巩兰菊 

机构地区：[1]河北农业大学植物保护学院,河北省农作物病虫害生物防治工程技术研究中心,保定071001 [2]保定学院生化系,保定071000 [3]河北省曲周县农牧局,曲周057250

出　　处：《植物保护》2012年第5期78-83,共6页Plant Protection

基　　金：现代农业产业技术体系(CARS-14)

摘　　要：对从土壤中分离到的金龟子绿僵菌中生长性状较好的6个菌株,通过测定对黄粉虫幼虫的僵虫率和致死中时,选出3株致病效果好的菌株M7-9、M7-(16-18)、M7-(64-65),再采用点滴法接种对斜纹夜蛾进行毒力测定。结果表明,不同菌株对斜纹夜蛾的毒力与菌株种类有密切关系,3株菌株对斜纹夜蛾的LT50分别为2.70、3.07、5.79d。以此3株菌进行抗高温及抗紫外线试验,菌株M7-9和M7-(16-18)对高温和紫外线都具有较好的抗性,且M7-9对紫外线的抗性强于M7-(16-18),两菌株均具有进一步研究的价值。Six strains of Metarhizium anisopliae with good growth traits,isolated from soil in Baoding area,were screened against Tenebrio molitor Linnaeus by bioassay.Among them,M7-9,M7-（16-18）and M7-（64-65）strains,which showed higher virulence than other isolates,were selected to test their pathogenicity against Spodoptera litura（Fabricius）.The LT50 of strain M7-9,M7-（16-18） and M7-（64-65） against S.litura were 2.70,3.07 and 5.79 d,respectively.The 3 strains were then treated with the ultraviolet ray and heat.The results demonstrated that the resistance of M7-9 and M7-（16-18） to high temperature and ultraviolet ray was higher,and the resistance of M7-9 to the ultraviolet ray was higher than that of M7-（16-18）.These results suggested that strains M7-9 and M7-（16-18） have better exploitation and application value in the future.

关 键 词：金龟子绿僵菌 斜纹夜蛾 生物测定 筛选 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]