阿司匹林预处理对大鼠局灶性脑缺血/再灌注后IL-6、SOCS-3mRNA表达的影响  

Effect of aspirin pretreatment on IL-6 and SOCS-3mRNA expression levels in focal cerebral ischemia/reperfusion

作　　者：李玲玲[1] 唐兴江[2] 牛智领 

机构地区：[1]四川石油管理局总医院,四川成都610213 [2]泸州医学院神经内科,四川泸州646000 [3]濮阳市第一人民医院,河南濮阳457000

出　　处：《西部医学》2012年第10期1858-1862,共5页Medical Journal of West China

摘　　要：目的探讨阿司匹林(Aspirin,ASA)预处理后,对大鼠局灶性脑缺血/再灌注(cerebral ischemia/reperfu-sion,CI/RP)缺血脑组织保护作用的可能机制。方法将雄性健康SD大鼠95只随机分为假手术组、缺血对照组、小剂量ASA组(10mg/kg)、大剂量ASA组(150mg/kg)。采用改良线栓法制作大鼠CI/RP。术后分别在6、12、24、72h和7d时间点进行神经功能评分,白细胞介素6(IL-6)、细胞因子信号转导抑制因子(SOCS-3mRNA)含量检测,用术后24h的大鼠进行TTC染色计算脑梗死体积。结果阿司匹林预处理后,小、大剂量ASA组大鼠神经功能评分较对照组明显升高(P<0.05),24h梗死病灶体积与对照组比较分别减少54.48%和30.90%,病灶侧脑组织IL-6含量较对照组明显下降(P<0.05),SOCS-3mRNA含量较对照组明显上升(P<0.05),在术后12h～3d与对照组相比较均有显著性差异(P<0.05)。结论 ASA药物预处理对脑缺血/再灌注损伤有保护作用,其可能机制与抑制IL-6的产生和上调SOCS-mRNA表达有关,小剂量ASA作用优于大剂量的ASA。Objective After aspirin（ASA） pretreatment,the changes dyna-mically of the ischemic brain tissue IL-6（IL-6） and cytokine signal transduction inhibitor-3（SOCS-3mRNA） mRNA expression were studied after rat focal cerebral ischemia/reperfusion（CI/RP）,so as to clarify the role of aspirin,the possible mechanism of brain protection.Methods 95 male healthy SD rats were randomly divided into sham-operated group,ischemic control group,low-dose ASA group（10mg·kg^-1） and high-dose ASA group（150mg·kg^-1）.Modified suture method was used rat focal cerebral ischemia / reperfusion model.At 6h,12h,24 h,72h,7d time rats were tested neurological score.Taking ischemic cerebral hemisphere was measured in brain tissue IL-6 and SOCS-3mRNA content,and the surgical rats after 24h were used for TTC staining to infarct volume.Results After aspirin pretreatment,the neurological score of Small-dose and high-dose ASA group were significantly higher than that of the control group.24h after infarct,the lesion volume decreased by 54.48%,30.90% compared with the control group.The two experimental group＇s side of the brain lesions IL-6 levels decreased significantly compared with the control group.The two experimental groups side of the brain lesions SOCS-3mRNA levels significantly increased compared with the control group.Conclusion Aspirin pretreatment has effect to perfect the MCA-CI/RP neurological deficits.The possible mechanism and inhibition of inflammatory cytokines IL-6 production and increases SOCS-mRNA related.low dose of ASA is superior to high-dose ASA group.

关 键 词：阿司匹林 脑缺血再灌注 白细胞介素-6 细胞因子信号转导抑制因子-3mRNA 

分 类 号：R33[医药卫生—人体生理学]