多菌灵对新疆灰漠土细菌群落多样性的影响  

Effect of carbendazim on bacterial community diversity of grey desert soil in Xinjiang

作　　者：肖丽[1] 冯燕燕[2] 赵靓[2] 周川[2] 冶军[2] 危常州[2] 侯振安[2] 

机构地区：[1]新疆农业科学院吐鲁番农业科学研究所,新疆吐鲁番838000 [2]石河子大学农学院农业资源环境系,新疆石河子832003

出　　处：《干旱地区农业研究》2012年第5期147-153,共7页Agricultural Research in the Arid Areas

基　　金：国家科技支撑计划课题(2007BAC20B04)

摘　　要：本研究旨在探明不同浓度多菌灵处理对土壤细菌群落多样性的影响，从而为多菌灵的合理使用和减少环境污染提供科学依据。采用PCR—DGGE方法，研究了新疆灰漠土上不同浓度多菌灵处理（O、5、10、20、50mg／kg和100mg／kg）细菌群落结构的变化。结果表明：试验条件下，在土壤细菌群落多样性分析中，Shannon—Wiener指数和丰富度在多茵灵施用14d时随着多菌灵浓度的增加有所增加，在28d和42d时则随着多茵灵浓度的增加而降低，shannon—wiener指数降低的幅度分别为4．05％一29．48％和6．13％一16．86％，丰富度降低的幅度分别为9．01％一63．64％和22．22％～38．89％；多菌灵处理对细菌均匀度和Simpson指数的影响不大。综合分析认为，施用多菌灵不仅影响了土壤细菌群落的种类和结构组成，还改交了土壤细菌的种群数量和结构多样性，导致其多样性降低。The aim is to explor the effect of carbendazim on bacterial community diversity of grey desert soil, and to provide the scientific basis for the safe handing of carbendazim in Xinjiang. Using the PCR - DGGE method, the effects of carbendazim at different concentrations （0, 5, 10, 20, 50 mg/kg and 100 mg/kg dry soil） on bacterial community di- versity of grey desert soil in Xinjiang were studied. Under experimental conditions, the variety and structure of bacterial community were changed by adding carbendazim in soil. With carbendazim concentration increasing, the Shannon-Wiener index and abundance index were increased at 14th day, but decreased by 4.05% ～ 29.48% and 9.01%～63.64% re spectively at 28th and by 6.13 % ～ 16.86% and 22.22% ～38.89 % respectively at 42nd day.. Treatments with car bendazim had little effects on evenness and Simpson index of soil bacteria. According to comprehensive analysis, the ap plication of carbendazim affected the types and structures of soil bacteria, and changed the diversity of population and structure of soil bacteria, which led to the bacterial community diversity decreasing.

关 键 词：多菌灵 灰漠土 细菌 遗传多样性 PCR—DGGE 

分 类 号：S154.3[农业科学—土壤学]