气相色谱法测定毛支清口服液中甲基正壬酮含量  

Content Determination of Methyl Nonyl Ketone in Maozhiqing Oral Liquid by Gas Chromatography

作　　者：乌日图[1] 徐晓丽 刘世杰[1] 魏文静 刘同祥[1] 

机构地区：[1]中央民族大学中国少数民族传统医学研究院,北京100081 [2]河南太龙药业股份有限公司,郑州450001

出　　处：《中国实验方剂学杂志》2012年第21期64-66,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81073151)

摘　　要：目的:建立毛支清口服液中甲基正壬酮的含量测定方法。方法:采用气相色谱法。Rtx-1毛细管色谱柱(0.25mm×30 m,0.25μm),FID检测器,柱温80℃(2 min),以10℃.min-1升至220℃,再以20℃.min-1升至270℃;进样口温度240℃,检测器温度280℃,进样量1μL,分流比30∶1,载气为氮气,柱流量1.13 mL.min-1,空气流量400 mL.min-1;氢气流量40 mL.min-1。结果:甲基正壬酮进样浓度为0.032 1～0.641 6 g.L-1时与峰面积呈良好的线性关系,回归方程为Y=3.00×106X-19 844(r=0.999 8),平均回收率97.76%,RSD 1.82%。结论:所建立的气相色谱方法简便、准确、灵敏度高、重复性好,可作为毛支清口服液的质量控制标准。Objective： To establish an method for determination of methyl nonyl ketone in Maozhiqing Oral Liquid. Method： The methyl nonyl ketone was determined by gas chromatography with Rtx-1 capillary chromatographic column （0.25 mm x30 m, 0.25 μm）. Column temperature： 80 ℃ for 2 min, increased at 10℃.min-1 to 220 ℃, then 20 ℃·min^（-1） to 270 ℃. Injector temperature： 240 ℃, FID temperature： 280 ℃. Injector volume was 1 μL; Split ratio was 30： 1. Nitrogen was used as carrier gas, the flow of column was 1.13 mL·min-1 Air： 400 mL .min-1 Hydrogen： 40 mL .rain-1 Result： Methyl nonyl ketone has a good linear relationship with the peak area within the range of 0. 032 143.641 6 g ·L-1 with an regression equation being Y = 3.00 × 10^6X - 19 844 （r = 0. 999 8）. The average recovery was 97.76% and the relative standard deviation was 1.82%. Conclusion： The established method of gas chromatography can be used for quality control of Maozhiqing Oral Liquid because of its simplicity, accuracy, high sensitive and good reproducibility.

关 键 词：毛支清口服液 甲基正壬酮 气相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]