pH、无机盐和有机溶剂对美洲大蠊醇提取物中蛋白质稳定性的影响  

Stability of Protein of Ethanol Extract from Periplaneta americana with pH,Inorganic Salt and Organic Solvent

作　　者：吴少辉[1] 罗廷顺[1] 张成桂[1] 巫秀美[1] 刘光明[1] 

机构地区：[1]大理学院药学院,云南大理671000

出　　处：《中国实验方剂学杂志》2012年第21期74-77,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30560181);云南省重点产业创新工程基金项目(2008IF012)

摘　　要：目的:对美洲大蠊醇提取物中蛋白质稳定性进行研究。方法:通过采用UV法和Folin-酚试剂法,考察了pH、无机盐和有机溶剂等影响因素对蛋白质稳定性的影响。结果:pH、氯化钠、硫酸铵、乙腈和甲醇随着剂量的增加,在不同程度上破坏了蛋白质的结构。结论:不同的影响因素通过改变蛋白质物理化学性质,破坏了蛋白质的稳定性。Objective： Studying the stability of the protein of atitumor activity from Periplaneta americana. Method： The factors of pH, inorganic salt and organic solvent on stability of the protein were studied by the UV and Lowrry method. Result： pH, sodium chloride, ammonium sulfate, acetonitrile and methanol are added and the structure of protein is changed. Conclusion： The physical and chemical properties are changed by different kinds of factors, and finally the stability of the protein is damaged.

关 键 词：Folin-酚试剂法 美洲大蠊 紫外分光光度法 

分 类 号：R284.1[医药卫生—中药学]