新疆道地药材伊贝母HPLC-ELSD指纹图谱研究及西贝母碱的含量测定  

Study on HPLC-ELSD Fingerprints of Bulbus Fritillariae Pallidiflorae and the Determination of Sipeimine in Them

作　　者：蔡晓翠 贺金华 徐芳 赵军 毛艳 戎晓娟 康雨彤 凯撒.苏莱曼 

机构地区：[1]新疆维吾尔自治区药物研究所,乌鲁木齐830004 [2]新疆维吾尔自治区中药民族药研究所,乌鲁木齐830002

出　　处：《中国实验方剂学杂志》2012年第21期82-85,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：新疆维吾尔自治区重大专项项目(200733146-1)

摘　　要：目的:建立新疆道地药材伊贝母HPLC-ELSD指纹图谱及西贝母碱的含量测定方法。方法:采用HPLC-ELSD法测定了10批伊贝母药材样品。色谱条件:Cosmosil C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.03%二乙胺溶液,梯度洗脱,流速1.0 mL.min-1,柱温30℃,进样量20μL;ELSD检测器,漂移管温度67℃;气体流速2.0 L.min-1。结果:建立了不同批次伊贝母的HPLC-ELSD指纹图谱,并进行相似度比较,对伊贝母中西贝母碱进行了含量测定。结论:该方法操作简便、重复性好,建立的伊贝母指纹图谱和西贝母碱的含量测定方法可用于伊贝母的质量控制。Objective： To establish HPLC-ELSD fingerprints of Bulbus Fritillariae Pallidiflorae and determine the content of sipeimine in them. Method： The HPLC-ELSD fingerprints of Bulbus Fritillariae Pallidiflorae from different places were obtained. The HPLC separation was performed on a Cosmosil Cls column （4.6 mmx 250 ram, 5 p.m） in gradient elute mode with a mixture consisting of acetonitrile and 0.03% diethylamine solution at a flow rate of 1.0 mL .min-1. The ELSD detector is used and the drift tube temperature of ELSD was set at 67 ℃ with the flow rate of 2.0 L .rain-t. Result： The HPLC-ELSD fingerprints were set up and the similar degrees to the crude drugs collected in different places were compared. The content of sipeimine was determined. Conclusion： The method is simple and reproducible and can be used for the quality control of Bulbus Fritillariae Pallidiflorae.

关 键 词：伊贝母 西贝母碱 指纹图谱 HPLC—ELSD 

分 类 号：R284.1[医药卫生—中药学]