近红外光纤光谱法快速检测清热解毒口服液中黄芩苷的含量  

Determination of Baicalin of Qingre Jiedu Oral Solution by Near-infrared Fibre-optical Spectroscopy Rapidly

作　　者：白雁[1] 李珊[1] 龚海燕[1] 

机构地区：[1]河南中医学院,郑州450008

出　　处：《中国实验方剂学杂志》2012年第21期109-112,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省重大公益科研项目(081100912500);河南省杰出人才项目(084200510017)

摘　　要：目的:建立应用近红外光纤光谱法对清热解毒口服液进行现场快速定量分析的方法。方法:收集100批清热解毒口服液为样本,利用HPLC测定样品中黄芩苷的含量,采集光谱,用偏最小二乘法(PLS)对清热解毒口服液中黄芩苷成分建立了定量分析模型,并对模型的处理方法、波长范围进行了研究。结果:建立的黄芩苷校正模型相关系数(R2)、估计误差均方根(RMSEE)、相对分析误差(RPD)分别为0.915 4,0.040 3,3.44。经外部验证,校正模型的预测均方差(RMSEP)、平均回收率分别为0.039 9,100.299%。结论:本研究使用近红外光纤光谱法所建立的对清热解毒口服液中黄芩苷的分析方法快速、准确,为清热解毒口服液的现场、即时快速定量分析提供了一种新方法,同时也为生产过程中的在线质量监控奠定了基础。Objective： To establish a new method for on-site rapidly analyzing Qingre Jiedu Oral Solution by near-infrared fibre-optical spectroscopy. Method： The samples are 100 batches of Qingre Jiedu Oral Solution. Baicalin in Qingre Jiedu Oral Solution was examined by HPLC, and then, scaning spectral. Quantitative analysis model of baicalin in Qingre Jiedu Oral Solution was established using partial least squares （PLS）. The model conditions was researched, such as different spectral pretreatments and wavelength regression. Result： The correlation coefficients （R2） , the root-mean-square error of estimated （RMSEE） and the RPD of the calibration model for baicalin were 0. 915 4, 0.040 3 and 3.44, respectively; the root-mean-square error of prediction （RMSEP） and the average rate of recovery were 0. 039 9 and 100. 299%. Conclusion： The analysis method based on near-infrared fibre-optical spectroscopy provides a foundation for rapid quantitative analysis of Qingre Jiedu Oral Solution, which sets up the foundation of product-line control of Qingre Jiedu Oral Solution and can be used for on-site rapid testing of drugs.

关 键 词：近红外光纤光谱法 偏最小二乘法 清热解毒口服液 黄芩苷 快速测定 

分 类 号：R284.1[医药卫生—中药学]