栀子苷的快速提取分离及其镇痛抗炎作用研究  被引量:25

Fast Extraction of Geniposide and its Analgestic and Anti-inflammatory Effect

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作  者:张文娟[1,2] 李茂星[1,2] 张泉龙[2] 邱建国[2] 尉丽力[2] 贾正平[1,2] 

机构地区:[1]兰州大学药学院,兰州730000 [2]兰州军区兰州总医院药剂科,全军高原环境损伤防治重点实验室,兰州730050

出  处:《中国实验方剂学杂志》2012年第21期170-174,共5页Chinese Journal of Experimental Traditional Medical Formulae

基  金:国家科技部重大专项(2008ZXJ09004-XXX);甘肃省科技重大专项计划项目(1102FKDA012);兰州军区总医院科研项目(院[2011]185号)

摘  要:目的:快速提取分离栀子苷单体,研究其体内镇痛、抗炎作用。方法:大孔吸附树脂初步纯化后,采用中压柱层析系统从中药栀子中快速提取分离栀子苷单体。将SPF级BALB/c小鼠分为5组,分别为对照组(生理盐水),栀子苷50,100,200 mg.kg-1组,吗啡或阿司匹林组(5,100 mg.kg-1)。小鼠尾静脉注射一次给药后,采用醋酸扭体实验、热板实验、二甲苯耳肿胀实验,角叉菜胶足肿胀实验,评价分离得到的栀子苷单体的体内镇痛、抗炎作用。结果:大孔吸附树脂初步纯化后得到栀子苷含量为78.96%,中压柱层析纯化后,栀子苷单体含量提高到98.47%。与对照组比较后得到,提取出的栀子苷能显著减少由腹腔注射醋酸引起的小鼠扭体次数(P<0.05),抑制二甲苯引起的耳肿胀(P<0.05)以及角叉菜胶引起的足肿胀(P<0.05),并呈现剂量依赖性。对小鼠热板痛阈值无明显影响。结论:实现了栀子苷的快速制备分离,分离获取的栀子苷具有较好的体内镇痛、抗炎作用。Objective: To extract and separate the geniposide monomer rapidly, and study its analgesic and anti-inflammatory effects. Method: Macroporous resin was used to rich and isolate geniposide from Fruit Gardenia jiaminoides and medium pressure column chromatography system was used to purify geniposide monomer quickly. BALB/c mice were divided into 5 groups: normal control group (saline) , geniposide groups (50, 100, 200 mg ·kg-1) and morphine group or aspirin group (5, 100 mg ·kg-1). Administrated by tail vein injection, hot plate test, acetic writhing test, xylene ear swelling test and the carrageenan paw edema test were used to evaluate the analgesic and anti-inflammatory activities of geniposide monomer in mice. Result: The content of geniposide was raised to 78.96% with macroporous resin, and 98.47% with medium-pressure column chromatography purification. Geniposide monomer could significantly reduce the writhing actions, which were induced by the intraperitoneal injection of acetic acid in mice, inhibit the xylene-induced ear swelling and carrageenan-induced paw swelling with a dose-dependent manner (P 〈 0.05), but it could not increase the hot plate pain threshold. Conclusion: Geniposide was separated and purified rapidly and successfully and it exhibited good analgesic and anti-inflammatory effects in mice.

关 键 词:栀子苷 提取分离 镇痛 抗炎 

分 类 号:R285.5[医药卫生—中药学]

 

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