清浊安中汤对慢性胃炎脾胃湿热证大鼠模型细胞凋亡及Bcl-2的影响  

Influence of Qingzhuo Anzhong Decoction on Apoptosis and Bcl-2 in Rat Model with Splenogastric Hygropyrexia Syndrome of Chronic Gastritis

作　　者：李合国[1] 劳绍贤[2] 

机构地区：[1]河南中医学院第一附属医院,郑州450000 [2]广州中医药大学,广州510405

出　　处：《中国实验方剂学杂志》2012年第21期189-192,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30271572)

摘　　要：目的:探讨清浊安中汤治疗脾胃湿热证作用机制及脾胃湿热证大鼠模型建立。方法:50只大鼠随机分为正常对照组、胃炎脾虚证组、胃炎脾胃湿热证组、清浊安中汤高、低剂量组,以2%水杨酸钠、番泻叶100%水煎液10 mL.kg-1.d-1灌胃,连续20 d,复制胃炎脾虚证。采用"病证结合"方法,以2%水杨酸钠(10 mL.kg-1.d-1灌胃)+高脂(20%蜂蜜水)高糖(10g.kg-1.d-1)+人工气候箱复制脾胃湿热证大鼠模型并用清浊安中汤(2,1 g.kg-1.d-1)对脾胃湿热证大鼠模型防治,常规HE胃黏膜病理组织染色、TUNEL(原位末端标记法)检测细胞凋亡、SABC(免疫组化)法检测Bcl-2蛋白表达。结果:脾胃湿热证组10只大鼠均有轻-重度炎症,细胞凋亡指数0.236±0.021,Bcl-2蛋白表达0.247±0.041;脾胃湿热证大鼠模型表现出症状、体征与临床相符,胃黏膜炎症较正常组重而与脾虚证组无差异,细胞凋亡较脾虚组轻而Bcl-2蛋白表达大于脾虚组(P<0.05),清浊安中汤防治组对此有改善治疗作用。结论:大鼠模型符合脾胃湿热证属性,调节细胞凋亡和Bcl-2蛋白表达可能是清浊安中汤治疗脾胃湿热证作用机制之一。Objective： To study mechanism of Qingzhuo Anzhong decotion in treating splenogastric hygropyrexia syndrome （SHS） of chronic gastritis. Method： The 50 rats were randomly divided into normal control group, gastritis splenasthenie syndrome group, gastritis splenogastric hygropyrexia group, traditional Qingzhuo Anzhong decoction high dose, low dose 2, 1 g · kg^（-1） ·d^（-1） prevention and cure group. The rats were given corresponding drugs by intragastrie administration for 20 days. The gastritis splenasthenic syndrome model, gastritis damp-heat syndrome model, and gastritis damp-heat syndrome model were induced by mean of 2% sodium salieylate, honey, fats, senna leaf, the artificial climate box and Qingzhuo Anzhong decoction. The inflammation situation of gastric mucosa were observed by using gastroscope and common pathological staining method. The protein expression of gastric mucosa Bcl-2 was detected by immunohisochemistry. The cell apoptosis of gastric mucosa was detected by the home position terminal sign notation. Result： There all were lightly-hyperphlogosis, apoptotic index being 0. 236 ± 0. 021, Bel-2 proteinum expression 0. 247 ± 0. 041 in 10 rats of splenogastric hygropyrexia group. The gastritis damp-heat syndrome rat model appeared some symptoms and physical sign. The rat gastritis splenic asthenia group and gastritis moist heat group were quite serious in gastric mucosa inflammation, compared with the normal group, the difference was significant （P 〈 0.05）. In SHS group Bcl-2 protein expresses was more obvious than that in splenic asthenia group and the normal group （P 〈 0.05 ）. The cell apoptosis index of SHS group and splenasthenic syndrome group was larger than the normal group （P 〈 0.05） , but SHS group was lower than spleen asthenic syndrome group （P 〈 0.05）. Conclusion： Adjusting apoptosis, Bcl-2 protein expresses may be one of mechanisms of Qingzhuo Anzhong decoction.

关 键 词：慢性胃炎 脾胃湿热证 细胞凋亡 BCL-2 

分 类 号：R285.5[医药卫生—中药学]