六味地黄生物制剂对衰老模型小鼠脑海马区神经元Bcl-2和Bax mRNA表达的影响  

Effect of Liuwei Dihuang Decoction Metabolized by Photosynthetic Bacteria on the Expression of Bcl-2 and Bax mRNA in Hippocampus Neurons of Aging Model Mice

作　　者：丘婷[1] 李天河[1] 吴思[1] 陈朋[1] 赵越[1] 

机构地区：[1]广东药学院中药学院,广州510006

出　　处：《中国实验方剂学杂志》2012年第21期196-199,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省自然科学基金重点项目(825022401000006)

摘　　要：目的:观察六味地黄生物制剂(LW-PSB)对衰老模型小鼠脑海马区神经元Bcl-2和Bax mRNA表达的影响。方法:将昆明种小鼠随机分为模型组、阳性对照维生素E(VE 0.27 g.kg-1)组、LW-PSB治疗组(0.50 g.kg-1)和正常对照组,共4组,每组各10只。造模后第15天开始按0.02 mL.g-1体重灌胃给药,模型组、正常对照组灌胃等体积生理盐水,每日1次,连续灌胃30 d。末次给药30 min后处死并分别取各组小鼠脑海马体,采用RT-PCR法检测各组小鼠脑海马区神经元Bcl-2和Bax mRNA的表达。结果:与模型组比较,LW-PSB治疗组Bcl-2 2-△△Ct显著升高(P<0.01),阳性对照组和LW-PSB治疗组Bax 2-△△Ct显著降低(P<0.01),阳性对照组和LW-PSB治疗组Bcl-2/Bax比值显著升高(P<0.01);与阳性对照组比较,LW-PSB治疗组Bcl-2 2-△△Ct升高(P<0.05)、Bax 2-△△Ct降低(P<0.05)和Bcl-2/Bax比值显著升高(P<0.01)。结论:六味地黄生物制剂通过促进脑海马区神经元Bcl-2 mRNA的表达和抑制Bax mRNA的表达起到抗衰老作用。Objective： To investigate the effect of Liuwei Dihuang decoction metabolized by photosynthetic bacteria （LW-PSB） on .the expression of Bcl-2 and Bax mRNA in hippocampus neurons of aging model mice. Method ： Mice were randomly divided into 4 groups ： model group, positive control group （rE 0.27 g ·kg^（-1） ） , LW-PSB-treated group （0.50 g·kg^（-1） ） and normal group. Each group was administrated continuously for 30 days. After 30 rain of administration, the hippocampus of each group was separated and RT-PCR was adopted to detect the expression of Bcl-2 and Bax mRNA in hippocampus neurons. Result： Compared with the model group and positive control group, the Bcl-2 2^（-△△c1） and Bcl-2/Bax were increased and Bax 2^（-△△c1）was decreased in LW-PSB-treated group. Conclusion： LW-PSB might up-regulate expression of Bcl-2 mRNA and down-regulate expression of Bax mRNA to anti-aging.

关 键 词：六味地黄生物制剂 衰老 BCL-2 BAX 表达 

分 类 号：R285.5[医药卫生—中药学]