侗药五味止泻汤对溃疡性结肠炎大鼠组织热休克蛋白70 mRNA及NF-κB p65表达的影响  

Influences of Dongyao Wuwei Zhixie Decoction on Expression of HSP70 mRNA and NF-κB p65 in the Ulcerative Colitis Rats

作　　者：赖象权[1] 何本求[2] 杨斌[2] 肖成[1] 王子明[1] 朱东东[1] 杨倩[2] 

机构地区：[1]贵阳中医学院第一附属医院,贵阳550001 [2]贵阳中医学院,贵阳550002

出　　处：《中国实验方剂学杂志》2012年第21期203-207,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省中医药管理局课题(QZYY2011-02);贵州省贵阳市科技局项目(筑科合同[2011103]号)

摘　　要：目的:运用荧光实时定量PCR(real-time PCR)研究侗药五味止泻汤对溃疡性结肠炎大鼠结肠组织热休克蛋白(HSP70)mRNA和核因子(NF)-κB p65表达的影响,探讨其在发病中作用机制。方法:将60只SD大鼠随机分为正常组,模型组,侗药五味止泻汤低、中、高剂量组(5.5,11,22 g.kg-1)和美沙拉嗪(0.5 g.kg-1)组,共6组,每组10只。采用2,4,6-三硝基苯磺酸和乙醇灌肠法制备UC大鼠模型,观察各组大鼠结肠炎疾病活动指数(DAI),10 d后取结肠组织评价黏膜损伤程度,采用免疫组化染色(SABC法)检测结肠组织NF-κB p65表达情况;运用荧光实时定量PCR(real-time PCR)检测结肠组织中HSP70 mRNA的表达变化。结果:侗药五味止泻汤能降低大鼠结肠DAI及组织损伤评分(P<0.01),提高大鼠HSP70 mRNA水平(P<0.01),并且降低NF-κB p65的表达(P<0.01)。结论:侗药五味止泻汤治疗UC的作用机制可能与通过提高保护性蛋白HSP70 mRNA水平表达和抑制NF-κB p65的激活有关。Objective： To study the effect of Dongyao Wuwei Zhixie decoction on expressions of heat shock protein 70 （HSP70） mRNA and nuclear factor kappa B p65 （NF-KB p65） in ulcerative colitis rats and its mechanisms. Method： Sixty SD rats were randomly divided into normal group, model group, Dongyao Wuwei Zhixie decoction low, middle and high dose groups, Mesalazine group, 10 each group. 2, 4, 6-trinitrobenzene sulfonic acid （TNBS） /ethanol solution was administered by enema to make ulcerative colitis model. The activity index （ DAI ） and colonic mucosal injury degree were observed, NF-KB p65 was determined by immunohistochemical staining （SABC method） and colonic tissue HSP70 mRNA expression was detected real time fluorescent quantitative PCR （real-time PCR） 10 days later. Result： Dongyao Wuwei Zhixie decoction can significantly reduce the DAI sores and tissue damage score of the rats＇ colon, increase the HSP70 mRNA level, and decrease the expressions of NF-KB p65 in rats. Conclusion： Dongyao Wuwei Zhixie Decoction in the treatment of UC mechanism may be associated with the enhancement of protective protein HSP70 mRNA expression level and inhibition of NF- KB p65 activation.

关 键 词：侗药五味止泻汤 溃疡性结肠炎 HSP70MRNA 核因子-ΚBP65 荧光实时定量PCR 

分 类 号：R285.5[医药卫生—中药学]