清宫汤抗焦虑作用及其机制的实验研究  

Research on the Anti-anxiety Effects and the Mechanisms of Qinggong Decoction

作　　者：张世亮[1] 李越兰[1] 张丽英[1] 宋玉良[1] 

机构地区：[1]浙江中医药大学,杭州310053

出　　处：《中国实验方剂学杂志》2012年第21期215-217,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：2010年浙江省教育厅项目(Y201016975)

摘　　要：目的:观察清宫汤对高架十字迷宫小鼠行为学及脑神经递质的影响,探讨其抗焦虑作用及机制。方法:将雄性小鼠随机分为空白对照组(生理盐水)、地西泮对照组(0.001 5 g.kg-1)、清宫汤高、中、低剂量组(12,6,3 g.kg-1),每组10只。灌胃给药,每天1次,连续10 d后,采用高架十字迷宫焦虑动物模型,观察清宫汤对小鼠行为学的影响,酶联免疫吸附测定法(ELISA)检测小鼠脑神经递质含量。结果:清宫汤明显提高小鼠在高架十字迷宫装置中的开放臂进入次数比(OE%)和开放臂停留时间比(OT%);与地西泮组比较,清宫汤高剂量组OE%有显著差异(P<0.01),低剂量组OT%有显著差异(P<0.05)。脑神经递质测试,清宫汤明显降低5-羟色胺(5-HT),去甲肾上腺素(NE)浓度,其中中剂量组5-HT浓度降低显著(P<0.05),高剂量组NE浓度降低显著(P<0.01);与地西泮组比较,清宫汤各组5-HT,NE浓度无显著差异;多巴胺(DA)浓度各组无统计学差异。结论:清宫汤具有抗焦虑作用,其作用机制与降低小鼠脑组织中5-HT,NE浓度相关。Objective： To investigate the influence of Qinggong decoction （QGD） on behavior and neurotransmitter in the elevated plus-maze mice, and to explore the anti-anxiety effects and the mechanisms. Method： Fifty male mice were randomly divided into 5 groups： control group （normal saline） , diazepam group （0. 001 5 g ·kg^（-1））, QGD high-dose group （12 g ·kg^（-1））, medium-dose group （6 g ·kg^（-1）） and low- dose group （3 g ·kg^（-1）） , and gavage administration was carried out once per day for continuous 10 days. Then adopted anxiety animal model with the elevated plus-maze was used to observe the influence of QGD on behavior, and the concentration of brain neurotransmitter was detected in mice by ELISA. Result： QGD could increase the percentage of the times entering the open arms （OE%） and stay duration in the total period （OT%）; compared with diazepam group, the high-dose group had a significant difference in OE% , and low-dose group had a significant difference in OT%. In the neurotransmitter testing, QGD significantly lowered the concentration of 5- hydroxytryptamine （5-HT） and noradrenaline （NE）, and in which medium-dose group significantly reduced the concentration of 5-HT （P 〈 0.05） , and high-dose group reduced significantly concentration of NE （P 〈 0.01 ） ; compared with diazepam group, the concentration of 5-HT and NE of QGD groups had no obvious difference; the concentration of dopamine （DA） was not statistically different in groups. Conclusion： QGD has anti-anxiety effect, and the mechanism is related to lowering the concentration of 5-HT and NE in mice brain tissues.

关 键 词：清宫汤 高架十字迷宫 抗焦虑 神经递质 

分 类 号：R285.5[医药卫生—中药学]