参附益心颗粒对慢性心力衰竭大鼠血浆与心肌血管紧张素Ⅱ的影响  

Effect of Shenfu Yixin Granule on Circular and Myocardial Angiotensin ⅡLevel in Chronic Heart Failure Rats

作　　者：王永霞[1] 张群生 任红杰[1] 李彬[1] 朱明军[1] 

机构地区：[1]河南中医学院第一附属医院心脏中心,郑州450000 [2]洛阳市第一人民医院,河南洛阳471000

出　　处：《中国实验方剂学杂志》2012年第21期226-229,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省科技攻关计划项目(0624410031);"十一五"国家科技支撑计划(2007BAI20B075);河南省教育厅自然科学基金(2006360006)

摘　　要：目的:探讨参附益心颗粒对慢性心力衰竭大鼠血浆与心肌血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)水平的影响。方法:随机将150只雄性SD大鼠分为造模组130只与假手术组20只,造模组结扎左冠状动脉前降支,假手术组仅冠状动脉前降支下穿线不结扎。造模8周后,经超声测定大鼠左室射血分数(left ventricular ejection fraction,LVEF)<50%者随机分为5组(卡托普利组、氯沙坦组、参附益心颗粒(5.5,21.9 g.kg-1)2个剂量组、模型组],每组10只,另选假手术组大鼠10只。假手术组及模型组以纯净水ig,药物干预组将药物溶于纯净水中ig给药,每天1次,4周后检测LVEF、腹主动脉采血、取心肌组织检测AngⅡ。结果:参附益心颗粒21.9 g.kg-1组有明显改善LVEF作用,参附益心颗粒2个剂量组均明显降低血浆及心肌AngⅡ水平,且作用与卡托普利相当。结论:参附益心颗粒可改善心衰大鼠心功能,下调血浆及心肌AngⅡ水平,阻断RAS,可能起到延缓或改善心肌重塑的作用。Objective： To observe the effect of Shenfu Yixin granule on circular and myocardial angiotensin II （ Ang 11 ） level in chronic heart failure rats. Method： One hundred and fifty heathly male SD rats were randomly divided into sham-operated group （n =5） and model group （n = 130）. In model group the left anterior descending coronary artery was occluded, in sham operation group the sutures were passed under the left anterior descending coronary artery without ligation. After 8 weeks the modeling group was randomly assigned to 5 groups （n = 10 in each group） based on the value of left ventricular eijection fraction （LVEF） 〈 50% obtained by Color Doppler Ultrasound, such as Captopril group, Losartan group, two groups of Shenfu Yixin granule （5.5, 21.9 g ·kg^（-1）） , model group. All the medicine was given through gavage once a day for 4 weeks, the rats in model group and sham operation group （n = 10） were ig administered with equivalent sodium chloride once a day for 4 weeks. After 4 weeks LVEF was recorded with Color Doppler Ultrasound; the aortaventralis blood and cardiac muscular tissue were collected to detect index. Result： The LVEF was improved markedly in Shenfu Yixin granule （21.9 g·kg^（-1） ） group. Two Shenfu Yixin granule groups obviously decrease circular and myocardial angiotensin 1I level, the function was similar to Captopril. Conclusion： Shenfu Yixin granule can improve cardiac function in chronic heart failure （CHF） rats, decrease circular and myocardial angiotensin 11 leve to block renin angiotensin system （RAS） , maybe delay or improve cardiac remodeling.

关 键 词：参附益心颗粒 慢性心力衰竭 循环与心肌血管紧张素Ⅱ 

分 类 号：R285.5[医药卫生—中药学]