醒脑静合生脉注射液对大鼠脑出血后脑组织内水通道蛋白表达的影响  

Effect of Xingnaojing and Shengmai Injection on AQP4 after Intracerebral Hemorrhage in Rats

作　　者：张青[1] 谢晓丽[1] 王其新[1] 马承泰[1] 蒋艳霞[1] 王守彪[1] 隋爱华[1] 

机构地区：[1]青岛大学医学院附属医院,山东青岛266003

出　　处：《中国实验方剂学杂志》2012年第21期243-247,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山东省中医药管理局基金项目(2005-020)

摘　　要：目的:探讨醒脑静合生脉注射液对大鼠脑出血后脑组织内水通道蛋白-4(AQP4)表达的影响。方法:将SD大鼠随机分为脑出血组(ICH)、生理盐水组(NS)、醒脑静合生脉注射液治疗组(XNJSM)、水蛭素治疗组(HIR),每组10只大鼠。采用自体不凝血注入法复制脑出血模型,造模后6 h,XNJSM组ip醒脑静合生脉注射液2 mL.kg-1+10 mL.kg-1(按1∶5比例混合),1次/d,连续3 d;HIR组给予脑内注入水蛭素10 U(5μL)1次。术后72 h取脑组织,HE染色观察各组血肿周围神经细胞形态学改变,免疫组化法及免疫印迹(Western blot)法检测各组血肿周围脑组织AQP4的表达。结果:脑出血后72 h脑组织内AQP4阳性细胞及蛋白表达与NS组比较明显增加(P<0.05),XNJSM组、HIR组脑组织病理形态明显改善,脑组织AQP4表达减少,与ICH组比较差异均有显著性(P<0.05)。结论:醒脑静合生脉注射液能够有效抑制大鼠脑出血后AQP4蛋白的表达,减轻脑水肿,对脑出血后脑组织发挥保护作用。Objective： To study the effect of Xingnaojing and Shengmai injection on the expression of aquaporin-4 （AQP4） in rat brain tissue after intracerebral hemorrhage （ICH）. Method： The ICH model was induced by injecting self-blood. Forty male Sprague-Dawley （SD） rats were randomly divided into ICH group （n = 10）, saline group （NS, n = 10）, Xingnaojing and Shengmai injection group （XNJSM, n = 10）, hirudin group （HIR, n = 10）. Six hours after model establishment, rats in XNJSM were received Xingnaojing of 2 mL ·kg^（-1） and Shengmai of 10 mL ·kg^（-1）through intraperitoneal injection, once daily, the therapeutic course was 3 days; Hirudin （ 10 U in 5 μL） was injected into the hematoma in HIR. Hematoxylin and eosin staining was performed to examine perihematomal neurocyte morphological changes, and the expression of AQPg in perihematomal tissue was determined by immunohistochemistry and Western bloting respectively after 72 h. Result： AQP4 positive cells and protein expression in perihematomal tissue was increased at 72 h after ICH, with significant difference to those in NS （P 〈 0.05 ）, Compared with ICH, in XNJSM group and HIR group, perihematomal tissue morphological changes were improved obviously, and AQP4 expression was also decreased significantly （ P 〈 0. 05 ）. Conclusion ： XNJSM injection could play a neuroprotective effect by inhibiting the expression of AQP4 and alleviating cerebral edema after ICH.

关 键 词：脑出血 醒脑静合生脉注射液 水通道蛋白-4 脑水肿 

分 类 号：R285.5[医药卫生—中药学]