温肾通络止痛颗粒对去卵巢大鼠骨密度及骨代谢的影响  

Effect of Wenshen Tongluo Zhitong Granule on Bone Mineral Density and Bone Metabolism in Ovariectomized Rats

作　　者：孙福荣[1] 郭杨[1] 马勇[1] 周信[2] 许建安[3] 

机构地区：[1]南京中医药大学,南京210029 [2]南京医科大学附属江宁医院,南京211100 [3]江苏省中医院,南京210029

出　　处：《中国实验方剂学杂志》2012年第21期275-277,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：康缘中医药科技创新与奖励基金(HZ1004KY)

摘　　要：目的:观察温肾通络止痛颗粒对去卵巢大鼠骨密度及骨代谢的影响,探究其防治骨质疏松症的机制。方法:将90只雌性大鼠随机分成空白对照组,模型组,温肾通络止痛颗粒低、中、高剂量组(按生药量计为4,8,16 g.kg-1)及尼尔雌醇组(1 mg.kg-1)共6组,采用卵巢切除的方法制成骨质疏松动物模型,ig给药3个月后进行骨密度(BMD)、降钙素(CT)、骨钙素(BGP)及雌二醇(E2)等骨代谢指标的检测。结果:对照组、模型组、尼尔雌醇组、温肾通络止痛颗粒低、中、高剂量组BMD分别是(0.231 8±0.003 6),(0.198 8±0.003 9),(0.219 9±0.002 4),(0.201 1±0.000 9),(0.202 3±0.002 7),(0.220 2±0.002 1)g.cm-2;CT含量分别为(74.61±10.54),(48.33±13.72),(71.72±14.83),(56.76±12.58),(68.39±13.74),(75.29±15.63)ng.L-1;E2含量分别为(88.61±17.54),(28.45±11.39),(71.71±8.69),(68.11±12.63),(70.93±10.42),(75.21±9.37)ng.L-1。模型组及各治疗组BGP含量与空白对照组比较无显著差异。温肾通络止痛颗粒低、中、高剂量组股骨BMD,CT,E2含量升高,与模型组相比差异具有显著意义(P<0.05),以高剂量组效果最明显(P<0.01)。结论:温肾通络止痛颗粒能够明显提高去卵巢大鼠骨密度值,改善其骨代谢状况,从而起到抗骨质疏松作用。Objective： To investigate the effect of Wenshcn Tongluo Zhitong granule on osteoporosis in rat ovariectomized model, to explore its mechanism. Method： Ninety female rats were randomly divided into control group, model group, low-dose, medium-dose, high-dose Wenshen Tongluo Zhitong granule groups and nyestrid （CEE3） group. Animal models of osteoporosis was induced with the ovariectomized method, and intragastric administration was performed. The bone mineral density （BMD） , calcitonin （CT） , bone Gla protein （BGP） and estradiol （E2） after 3 months were measured. Result： The BMD content in the control group, model group, CEE3 group, low-dose, medium-dose, high-dose of Wenshen Tongluo Zhitong granule group was （0.2318_＋0.0036）, （0.1988±0.0039）, （0.219 9±0.0024）, （0.201 1 ±0.000 9）, （0.2023± 0.0027）, （0.2202±0.002 1） g·cm^（-2）, the CT content is （74.61±10.54）, （48.33±13.72）, （71.72± 14.83）, （56.76±12.58）, （68.39±13.74）, （75.29 ±15.63） ng .L-1, the BGP content is （5.95 ± 1.20）, （5.46±1.34）, （6.27±1.21）, （6.08±1.24）, （6.12±1.47）, （6.15±1.19）~g-L-l,theE2 eontentis （88.61±17.54）, （28.45 ±11.39）, （71.71 ±8.69）, （68.11 ±12.63）, （70.93 ±10.42）,（75.21 -＋9.37） ng ·L^（-1）. The BGP content in ovariectomized rats had no significant change in the model group, treatment groups and CEE3 group compared to the control group. The BMD and CT content in treatment groups significantly increased compared with the model group, and the high dose group improved most significantly （P 〈 0.01）. The E2 content in treatment groups significantly increased compared with the model group （P 〈 0. 01 ）. Conclusion： Wenshen Tongluo Zhitong granule can significantly increases bone mineral density and improve bone metabolism in ovariectomized rats, which plays the role of anti-osteoporosis.

关 键 词：骨质疏松 温肾通络止痛颗粒 骨密度 骨代谢 

分 类 号：R285.5[医药卫生—中药学]