α-硫辛酸对老年大鼠外周血细胞线粒体的抗氧化作用  

THE ANTIOXIDATIVE EFFECT OF α-LIPOIC ACID ON BLOOD CELL MITOCHONDRIA OF AGED RATS

作　　者：田芳[1] 赵鹏[2] 袁准[2] 孙宇立[3] 邵春海[1] 刘景芳[1] 

机构地区：[1]复旦大学附属华山医院,上海200040 [2]北京大学医学部公共卫生学院毒理学系,北京100191 [3]上海市疾病预防控制中心,上海200336

出　　处：《营养学报》2012年第5期462-465,共4页Acta Nutrimenta Sinica

摘　　要：目的探讨α-硫辛酸(α-LA)对老年大鼠线粒体活性氧及膜电位的影响。方法以18月龄老年大鼠为研究对象,6月龄大鼠作为青年对照。不同浓度硫辛酸灌胃饲养7 d、停止灌胃3 d、再次恢复灌胃7 d,用双氢罗丹明123(DHR123)标记外周血细胞线粒体活性氧,罗丹明123(Rh123)指示线粒体膜电位,借助流式细胞仪评价LA对老龄大鼠体内细胞活性氧及膜电位的改变。结果老年大鼠外周血细胞线粒体活性氧水平高于青年对照,线粒体膜电位低于青年对照。LA灌胃饲养7d后,实验组老年大鼠活性氧水平低于老年对照组(P<0.05),线粒体膜电位高于老年对照组(P<0.05)。停止灌胃3 d后,各实验组活性氧水平不同程度回升,高剂量组较明显;各实验组线粒体膜电位变化不明显。继续用LA灌胃7 d,各实验组活性氧水平不同程度下降,线粒体膜电位变化无统计意义。结论 LA能清除活性氧,稳定线粒体膜电位;LA对活性氧的抑制有浓度依赖性,对线粒体膜电位的稳定作用较持久。Objective To investigate the antioxidantive effect of α-lipoic acid（α-LA） on the mitochondria of elderly Sprague Dawley（SD） rats.Method Sprague Dawley rats of 18 mo old were fed diet with α-LA as the experiment group.The control group was fed with the same diet without LA.The experiment group was fed LA diet for 1 w,then ceased feeding for 3 d,and continued feeding for another week.In the last day of each stage,Rhodamine123（Rh123） was used as fluorescent probe to indicate mitochondrial membrane potential.Dihydrorho-damine123（DHR123） was used to indicate mitochondrial ROS.Fluorescent signal was detected and analyzed by flow cytometry.Results Mitochondrial ROS level was higher and membrane potential was lower in the experiment groups than the control group.The ROS level was increased in the LA feeding stage and dropped after ceasing.The mitochondrial membrane potential was increased in the first week and maintained in the subsequent stages.Conclusion LA scavenges ROS and protects mitochondria from oxidative damages,thus plays an important role in antioxidantive and anti-aging activities,which depend on its concentration and duration in vivo.

关 键 词：Α-硫辛酸 血细胞 线粒体 活性氧 流式细胞术 抗氧化 抗衰老 

分 类 号：R965[医药卫生—药理学]