芦笋花药愈伤组织诱导技术研究  

Preliminary Study on Anther Culture Callus Inducement of Asparagus

作　　者：张元国[1] 李芳[1] 包艳存 

机构地区：[1]山东省潍坊市农业科学院,山东潍坊261071

出　　处：《安徽农业科学》2012年第31期15142-15143,共2页Journal of Anhui Agricultural Sciences

基　　金：山东省农业良种产业化项目(wkns200202)

摘　　要：[目的]对芦笋花药愈伤组织诱导培养技术进行详细研究。[方法]以MS为基本培养基,附加外源激素对二倍体花药愈伤组织诱导技术进行研究。[结果]二倍体芦笋花蕾长度为1.5～2.0 mm时,其诱导效果最好;采用5%高糖浓度可显著抑制花药体细胞分裂和提高花粉愈伤组织诱导率;同一品种不同植株花药愈伤组织诱导率差异显著;适合于二倍体花药愈伤组织诱导的激素配比是1.0 mg/LNAA+1.0 mg/L BA。[结论]为芦笋的品种改良与选育奠定了基础。[Objective] To study anther culture callus inducement of asparagus in detail.[Method] The diploid anther callus inducement was studied by MS as basic medium with exogenous hormone.[Result] The length of the diploid bud was 1.5-2.0 mm which induced result best;The high sugar density of 5% can repress the anther somatic cell division remarkably and improved the inducement rate of pollens callus;The callus inducement rate of the same plant with different species differed greatly;The suitable hormone proportion of diploid anther callus was 1.0 mg/L NAA＋1.0 mg/L BA.[Conclusion] The study will lay a foundation for improvement and breeding of asparagus.

关 键 词：芦笋 花药培养 愈伤组织 诱导 

分 类 号：S644.2[农业科学—蔬菜学]