进境韩国兰花细菌性褐腐病菌的分离与鉴定  

Isolation and Identification of Erwinia cypripedii from Imported Korea Cymbidium

作　　者：厉艳[1] 王英超[1] 尼秀媚[1] 甘琴华[1] 封立平[1] 

机构地区：[1]山东出入境检验检疫局,山东青岛266001

出　　处：《安徽农业科学》2012年第31期15243-15244,共2页Journal of Anhui Agricultural Sciences

基　　金：国家质检总局科技计划项目(2010IK270)

摘　　要：[目的]鉴定进境韩国兰花细菌性褐腐病菌。[方法]从韩国进境的大花蕙兰植株病变叶片中分离到细菌菌株,并通过形态鉴定、培养特征、生理生化及16S rDNA序列分析和致病性测定对其进行了鉴定。[结果]确认该病菌为兰花细菌性褐腐病菌(Erwinia cypripe-dii),这是我国首次从进境兰花中检出该病害。[结论]为我国兰花细菌性褐腐病菌的预防及控制奠定了基础。[Objective] The aim was to identify Erwinia cypripedii from imported Korea Cymbidium.[Method] The pathogenic bacteria was isolated from imported Korea Cymbidium leaves,and then it was identified by morphological identification,cultural characteristics,physiological and biochemical reactions,16S rDNA gene sequences analysis and pathogenicity test.[Result] The isolated strain was identified as E.cypripedii,and it was the first interception in China.[Conclusion] The research result lays the foundation for the prevention and control of E.cypripedii in China.

关 键 词：大花蕙兰 兰花细菌性褐腐病菌 病原鉴定 

分 类 号：S682.31[农业科学—观赏园艺]