Open Tubular Microreactor with Enzyme Functionalized Micro- fluidic Channel for Amperometric Detection of Glucose  

作　　者：张蕾 曲平 盛金 雷建平 鞠烷先 

机构地区：[1]State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing,Jiangsu 210093,China

出　　处：《Chinese Journal of Chemistry》2012年第9期2145-2150,共6页中国化学（英文版）

摘　　要：A simple and efficient method using enzyme immobilized microfluidic channel as open tubular microreactor was designed for amperometric detection of glucose. The microreactor was composed of a polydimethylsilicone/ glass hybrid device with three reservoirs, a cooling cave and a 6 cm capillary with a sampling fracture as micro-channel. The microchannel was further modified by thermal polymerization, followed by covalently attaching with glucose oxidase. Through fracture sampling and electrochromatography separation, the production via enzymatic reaction was determinated by Pt electrode at the end of capillary. The linear range for the detection of glucose was 0.05--7.5 mmol·L-1 with detection limit of 23μmol.L-1 The inter-and intra-chip reproducibilities for determination of 2.5 mmol-L-1 glucose were 98.5% （n=5） and 96.0% （n=5）, respectively. With the advantage of flexible assembly, rapid efficiency, good stability and low-cost, this microreactor provided a potential platform for estab- lishing a portable enzyme-based chemical detection system in practical application.A simple and efficient method using enzyme immobilized microfluidic channel as open tubular microreactor was designed for amperometric detection of glucose. The microreactor was composed of a polydimethylsilicone/ glass hybrid device with three reservoirs, a cooling cave and a 6 cm capillary with a sampling fracture as micro-channel. The microchannel was further modified by thermal polymerization, followed by covalently attaching with glucose oxidase. Through fracture sampling and electrochromatography separation, the production via enzymatic reaction was determinated by Pt electrode at the end of capillary. The linear range for the detection of glucose was 0.05--7.5 mmol·L-1 with detection limit of 23μmol.L-1 The inter-and intra-chip reproducibilities for determination of 2.5 mmol-L-1 glucose were 98.5% （n=5） and 96.0% （n=5）, respectively. With the advantage of flexible assembly, rapid efficiency, good stability and low-cost, this microreactor provided a potential platform for estab- lishing a portable enzyme-based chemical detection system in practical application.

关 键 词：MICROREACTOR amperometric detection GLUCOSE enzymatic catalysis microfluidic channel 

分 类 号：O644.18[理学—物理化学] Q554[理学—化学]