西地那非对兔膀胱出口部分梗阻模型膀胱顺应性及内皮素1的影响  

Effects of sildenafil on bladder compliance and endothelin-1 in rabbit model of partial bladder outlet obstruction

作　　者：陈洪德[1] 叶雪挺[1] 张奕荣[1] 翁志梁[1] 李澄棣[1] 

机构地区：[1]温州医学院附属第一医院泌尿外科,325000

出　　处：《中华医学杂志》2012年第38期2720-2723,共4页National Medical Journal of China

摘　　要：目的探讨西地那非对兔膀胱出口部分梗阻动物模型膀胱顺应性及膀胱组织内皮素1（ET．1）含量的影响。方法成年雄性新西兰白兔24只，随机数字表法分为4组，每组各6只。A组为假手术空白组：行假手术后每日生理盐水灌胃；B组为假手术治疗组：行假手术后每日西地那非（10mg／kg）灌胃；C组为膀胱出13部分梗阻治疗组：行膀胱出口部分梗阻手术后每口西地那非（10mg／kg）灌胃；D组为膀胱出口部分梗阻空白组：行膀胱出口部分梗阻手术后每日生理盐水灌胃。16周后4组分别行膀胱尿动力学检查，并取膀胱标本称重，采用酶联免疫法测定其ET-1含量。结果A—D组膀胱排尿压力阈值分别为（10．6±2．0）、（11．6±2．7）、（14．0±4．2）、（20．4±6．1）cmH2O（1cmH，0=0．098kPa），D组明显高于A、B、C组（均P〈0．01）；4组膀胱顺应性分别为（2．75±0．51）、（2．78±0．46）、（4．98±2．15）、（1．22±0．25）ml／cmH20，D组明显低于A、B、c组（均P〈0．01），C组高于A、B组（均P〈0．01），A组与B组间差异无统计学意义（P〉0．05）；4组膀胱标本质量分别为（5．0±0．4）、（4．6±0．4）、（8．2±1．3）、（17．9±2．3）g，C、D组高于A、B组（均P〈0．01），D组高于C组（P〈0．01）；4组膀胱组织中ET-1含量分别为（72±19）、（69±18）、（76±21）、（106±29）pg,／g，D组高于A、B、C组（均P〈0．05）。结论每日西地那非能有效缓解兔膀胱出口部分梗阻对膀胱顺应性的损害，保护膀胱功能，并且此作用可能与降低梗阻后膀胱组织中ET-1浓度有关。Objective To explore the effects of sildenafil on bladder compliance and endothelin-1 in the rabbit model of partial bladder outlet obstruction. Methods A total of 24 adult male New Zealand white rabbits were randomly divided into group A, group B, group C and group D （n =6 each）. The rabbit model of partial bladder outlet obstruction was established in groups C and D while groups A and B underwent a sham operation. Daily sildenafil （ 10 mg/kg） was dosed to groups B and C by lavage. Daily normal saline was dosed similarly to groups A and D. Bladder urodynamic examinations were conducted in each group at Week 16. Then bladder was isolated and weighed from each group. And ET-1 in bladder tissue was measured by ELISA. Results Pressure thresholds for voiding （PT） in A - D groups were （ 10. 6 ± 2. 0）, （ 11.6 ±2. 7）, （ 14. 0 ＋4. 2） and （20.4 ±6. 1 ） cm H20 respectively. Compared with groups A, B and C, PT in group D was significantly higher （ all P 〈 0. 01 ）. Bladder compliance in 4 groups were （2. 75 ± 0. 51 ）, （2. 78 ± 0. 46 ）, （ 4. 98 ± 2. 15 ） and （ 1.22 ± 0. 25 ） ml/cm H20 respectively. Compared with groups A, B and C, bladder compliance was significantly lower in group D （ all P 〈 0. 01 ） . Bladder compliance in group C was higher than that in groups A and B （ both P 〈 0. 01 ）. The weights of bladder specimens in 4 groups were （ 5.0 ± 0. 4） , （4. 6 ± 0. 4）, （ 8. 2 ± 1.3 ） and （ 17.9 ± 2. 3 ） g respectively. Compared with groups A and B, the weights of groups C and D were significantly heavier （ all P 〈 0.01 ）. And the weight of group D was much greater than that of group C （ P 〈 0.01 ）. The contents of ET-1 in bladder tissue of 4 groups were （72 ± 19）, （69 ± 18）, （76 ± 21 ） and （ 106 ± 29） pg/g respectively. Compared with groups A, B and C, ET-1 in bladder tissue was significantly higher in group D （ all P 〈 0. 05 ）. Conclusions Daily sildenafil can effectively alleviate the damage of r

关 键 词：膀胱颈梗阻 磷酸二酯酶抑制剂 顺应性 内皮素 

分 类 号：R694[医药卫生—泌尿科学]