大鼠Kupffer细胞内外Sonazoid微泡超声辐照下的动态现象  

Dynamic Phenomena of Sonazoid Microbubbles in Cultures of Rats Kupffer Cells during Ultrasound Insonation

作　　者：刘广健[1] 吕明德[1] 森安史典 谢晓燕[1] 刘泳东[3] 徐作峰[1] 谢晓华[1] 王竹[1] 

机构地区：[1]中山大学附属第一医院超声波科,广东广州510080 [2]日本东京医科大学消化内科 [3]中山大学附属第一医院病理科,广东广州510080

出　　处：《中山大学学报（医学科学版）》2012年第5期571-576,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金青年基金(30901384)

摘　　要：【目的】探讨超声辐照对Kupffer细胞内外Sonazoid微泡运动和形态的影响。【方法】分离大鼠Kupffer细胞。使用倒置相差显微镜观测Kupffer细胞吞噬Sonazoid微泡的情况。并使用临床超声检查仪在不同声压下辐照,观察微泡在Kupffer细胞内外的动态现象。【结果】Kupffer细胞对Sonazoid微泡吞噬率为91%。游离微泡在机械指数(MI)0.21时由静止出现震动,产生震动的最佳MI为0.21～0.44,微泡破裂的声压阈值是1.03。MI=1.18是黏附微泡破裂的阈值。被吞噬微泡破裂的阈值为MI=1.37。【结论】Sonazoid微泡能够被体外培养的Kupffer细胞吞噬。超声辐照下,声压对Kupffer细胞内外Sonazoid微泡的运动和形态有明显影响作用。[ Objective ] To investigate the influence of ultrasound insonation to the shape and movement of Sonazoid microbubbles outside and inside of Kupffer cells. [ Methods ] Kupffer cells were isolated from rats. Phagocytosis of Sonazoid microbubbles were observed under inverted phase contrast microscope. Ultrasound insonation was performed using a clinical ultrasound scanner, phenomena of microbubbles outside and inside of Kupffer cells in an acoustic field with different acoustic pressure were observed. [ Results ] The phagocytosis rate of Sonazoid microbubbles by Kupffer cells was 91%. The thresholds in term of mechanical index （MI） for free microbubbles to begin oscillation, reach best oscillation, and destruction were 0.21, 0.44, and 1.03, respectively. The thresholds of destruction were 1.18 for adherent microbubbles, and 1.37 for phagocytosed microbubbles, respectively. [ Conclusions ] Sonazoid microbubbles could be phagocytosed by Kupffer cells in vitro. Acoustic pressure of ultrasound insonation affects the movement and the shape of Sonazoid microbubbles outside and inside of Kupffer cells obviously.

关 键 词：KUPFFER细胞 Sonazoid微泡 超声辐照 现象 

分 类 号：R445.1[医药卫生—影像医学与核医学]