地塞米松对肠缺血再灌注后继发肺损伤的保护作用  

Protective Effect of Dexamethasone on Lung Injury Secondary to Intestinal Ischemia-reperfusion

作　　者：宋伟峰[1] 朱琼芳[2] 李瑞珍[1] 张政[3] 

机构地区：[1]中山大学附属第三医院药剂科,广东广州510630 [2]中山大学附属第一医院麻醉科恢复室,广东广州510080 [3]中山大学附属第三医院麻醉科,广东广州510630

出　　处：《中山大学学报（医学科学版）》2012年第5期614-618,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科技计划项目(2011A080504003)

摘　　要：【目的】探讨地塞米松对肠缺血再灌注肺损伤的保护作用。【方法】35只雄性昆明鼠随机平均分为5组,每组7只,分别是假手术组(S);模型组(II/R);模型+地塞米松预处理组(II/R+DP);模型+地塞米松缺血时处理组(II/R+DI);模型+地塞米松再灌注即刻处理组(II/R+DR)。每组实验结束之后留取肺标本,以待进行肺组织病理检测、MDA含量和SOD活性检测、TNF-α检测、NO含量测定、iNOS活性测定。【结果】肠缺血再灌注过程显著地造成了急性肺损伤,体现在病理积分显著降低(P<0.05,II/R vs.S),MDA、TNF-α、NO的含量以及SOD和iNOS活性的上升(P<0.05,II/R vs.S),地塞米松预处理能减轻肠缺血再灌注肺损伤(P<0.05,II/R+DP vs.II/R),而地塞米松缺血期和再灌注即刻处理对肺无明显保护作用。【结论】地塞米松预处理能减轻肠缺血再灌注继发性损伤,缺血期和再灌注即刻用药则无保护作用。[ Objective] To investigate the protective effect of dexamethasone on lung injury secondary to intestinal ischemia- reperfusion. [ Method ] Thirty-five male rats weighting 20-24 g were randomized into sham group, sole ⅡR group （ⅡR）, or ⅡR and preconditioning with dexamethasone （Ⅱ/R＋DP）, or Ⅱ/R＋DI group （respectively treated with dexamethasone after ischemia began）, or Ⅱ/R＋DR group （respectively treated with dexamethasone as soon as reperfusion）. At the end of experiment, lung tissue was obtained for histologic assessment and for the measurement of the levels of MDA, SOD, TNF-c~, NO, and iNOs. [Result] ⅡR resulted in lung injury evidenced as significant decreases in lung histological scores （P 〈 0.05, ⅡR vs. S）, accompanied with concomitant increases of the levels of MDA, SOD, TNF-α, NO and the activity of iNOs （P 〈 0.05, Ⅱ/R vs. S）. Preconditioning with dexamethasone attenuate ⅡR mediates lung injury （P 〈 0.05, Ⅱ/R＋DP vs. Ⅱ/R）, while treated with dexamethasone during the ischemia and reperfusion period were unprofitable to lung. [ Conclusion] Preconditioning with dexamethasone attenuate ⅡR mediates lung injury, while treated with dexamethasone during the ischemia and reperfusion period were unprofitable to lung.

关 键 词：地塞米松 肠缺血 再灌注 肺损伤 

分 类 号：R459.1[医药卫生—治疗学]