Electroacupuncture upregulated platelet derived growth factor expression in spared dorsal root ganglion of cats  

作　　者：Xifeng Wang Lianshuang Zhang Xiaobo Xu Wei Zhao Guixiang Liu 

机构地区：[1]Intensive Care Unit of Yantai Yuhuangding Hospital, Yantai 264000, Shandong Province, China [2]Department of Histology and Embryology, Binzhou Medical College, Yantai 264003, Shandong Province, China [3]Neuroscience Department, University of Southem Califomia, CA 90007, USA

出　　处：《Neural Regeneration Research》2012年第27期2131-2135,共5页中国神经再生研究（英文版）

基　　金：supported by the Natural Science Foundation of Shandong Province,No.ZR2011HQ048

摘　　要：A bilateral spared dorsal root ganglion model was established in healthy adult cats by bilateral resection of L1-5 and L7-S2 dorsal root ganglia. L6 dorsal root ganglia were spared. Zusanfi （ST36） and Xuanzhong （BL39） or Futu （ST32） and Sanyinjiao （SP6） were alternatively electro-stimulated on the right leg. Immunohistochemical staining of anti-serum platelet-derived growth factor demonstrated that the number of total neurons and medium-small sized platelet-derived growth factor positive neurons was significantly decreased on the 7~ day following injury. After 7 days of acupuncture, the total number of positive and large neurons staining for platelet-derived growth factor on the acupuncture side significantly increased compared to the non-acupuncture side. After acupuncture for 14 days, the total positive and medium-small sized neurons significantly increased compared with the non-acupuncture side. Results indicate that acupuncture promoted the synthesis of platelet-dedved growth factor in spared dorsal root ganglia.A bilateral spared dorsal root ganglion model was established in healthy adult cats by bilateral resection of L1-5 and L7-S2 dorsal root ganglia. L6 dorsal root ganglia were spared. Zusanfi （ST36） and Xuanzhong （BL39） or Futu （ST32） and Sanyinjiao （SP6） were alternatively electro-stimulated on the right leg. Immunohistochemical staining of anti-serum platelet-derived growth factor demonstrated that the number of total neurons and medium-small sized platelet-derived growth factor positive neurons was significantly decreased on the 7~ day following injury. After 7 days of acupuncture, the total number of positive and large neurons staining for platelet-derived growth factor on the acupuncture side significantly increased compared to the non-acupuncture side. After acupuncture for 14 days, the total positive and medium-small sized neurons significantly increased compared with the non-acupuncture side. Results indicate that acupuncture promoted the synthesis of platelet-dedved growth factor in spared dorsal root ganglia.

关 键 词：platelet-derived growth factor ACUPUNCTURE dorsal root ganglion IMMUNOHISTOCHEMISTRY CAT traditional Chinese medicine neural morphology neural regeneration 

分 类 号：Q516[生物学—生物化学] Q424