中药复方护骨胶囊对糖皮质激素诱导骨质疏松大鼠骨密度和骨形态计量学的研究  

Effect of Hugu capsule on BMD and histomorphometry in glucocorticoid-induced osteoporotic rats

作　　者：王晓东[1] 董群伟[1] 洪曼杰[1] 孙平[1] 胡伶平[1] 刘忠厚 

机构地区：[1]广东药学院附属第一医院骨科,广州510080 [2]中国老年学学会骨质疏松委员会

出　　处：《中国骨质疏松杂志》2012年第10期880-882,879,共4页Chinese Journal of Osteoporosis

摘　　要：目的探讨中药复方护骨胶囊(主要由制何首乌、淫羊藿、熟地黄等多味中药加工而成的复方制剂)对糖皮质激素诱导骨质疏松大鼠骨量丢失的影响。方法 3月龄SPF级雄性SD大鼠30只,随机平均分为3组:正常对照组(Nrm)、激素组(Met)和中药组(CH)。Met组:皮下注射甲强龙(Met)5mg/(kg.d),每周5次;CH组:在Met组基础上给予中药复方护骨胶囊(150 mg/(kg.d)灌胃,实验期12 w。大鼠右侧股骨和L5行骨密度(BMD)测定,右侧胫骨行骨形态计量学分析。结果 Met组L5和股骨BMD显著低于Nrm组;CH组L5和股骨BMD显著高于Met组。Met组Tb.N、%Tb.Ar、MS/BS、MAR和BFRs显著低于Nrm组,Tb.Sp、ES/BS显著高于Nrm组;中药组Tb.N、%Tb.Ar、MS/BS、MAR和BFRs显著高于Met组,Tb.Sp,ES/BS显著低于Met组。结论中药复方护骨胶囊在提高激素诱导骨质疏松大鼠的骨密度,促进骨形成,降低骨吸收,延缓骨量丢失方面有积极作用,对继发性骨质疏松的预防和治疗有一定前景。Objective To investigate the effect of Hugu capsule （ CH ）, mainly made of several kinds of traditional Chinese medicine including radix polygonum muhiflorum preparataon, herba epimedii, and prepared rehmannia root, on bone loss in osteoporotic rats induced by glueocorticoid. Methods Thirty 3- month-old male Sprague-Dawley rats were divided into 3 groups randomly： age-matched normal control group （Nrm） , methylprednisolone group （Met） , and Chinese medicine group （CH）. The rats in Met group were given a subcutaneous injection of methylprednisolone （Met） with a dose of 5.0 mg/kg per day, 5 times per week. The rats in CH group were not only given the same treatment in Met group, but also given a garage of Hugu capsule with a dose of 150 mg/kg per day. The experiment lasted for 12 weeks. BMD of the right femur and L5 vertebrae was tested. Histomorphometry was performed on the right tibia. Results BMD of the fight femur and I5 vertebrae in Met group was significantly lower than that in Nrm group. BMD of the right femur and 15 vertebrae in CH group was significantly higher than that in Met group. Tb. N, % Tb. At, MS/BS, MAR, and BFRs in Met group were significantly lower than those in Nrm group. Tb. Sp and ES/BS in Met group were significantly higher than those in Nrm group. Tb. N, % Tb. At, MS/BS, MAR, and BFRs in CH group were significantly higher than those in Met group. Tb. Sp and ES/BS in CH group were significantly lower than those in Met group. Conclusion CH has a positive effect on improving BMD in rats with osteoporosis induced by glucocorticoid, promoting osteogenesis, reducing bone resorption, and suspending bone loss. It has a potent application on the prevention and treatment of secondary osteoporosis.

关 键 词：骨质疏松 糖皮质激素 骨密度 骨形态计量学 

分 类 号：R453[医药卫生—治疗学]