不同温度及pH值对人结肠腺癌细胞模型的影响  

Effects of Different Temperature and PH Value on Caco-2 Cell Model

作　　者：刘影[1] 李月婷[1] 查雨锋[1] 王静娴[1] 肖婷婷[1] 李靖[1] 董莉[1] 傅晓钟[1] 黄勇[1] 王永林[1] 

机构地区：[1]贵阳医学院药学院,贵州贵阳550004

出　　处：《贵阳医学院学报》2012年第5期465-468,共4页Journal of Guiyang Medical College

基　　金：国家自然科学基金(No.20962004;No.81060359);贵州省中药现代化科技产业研究开发专项[黔科合设字(2010)5019];贵州省社会发展攻关计划[黔科合SY(2010)3046号];贵州省科技厅攻关计划[黔科合GY字(2008)3028]

摘　　要：目的:建立人结肠腺癌细胞系(Caco-2)细胞模型并对其药物研究的方法进行初步探讨。方法:通过测定跨细胞膜电阻(TEER)、荧光素通透量及碱性磷酸酶(AKP)评价Caco-2细胞模型,用MTT法考察HBSS缓冲液在不同pH值、不同温度及不同时间条件下对Caco-2细胞的毒性。结果:各项指标的测定值符合要求,表明本实验建立的Caco-2细胞模型的完整性、紧密性和通透性良好;进行药物Caco-2细胞吸收潜性研究时,药物作用4 h之内对Caco-2细胞模型没有影响。结论:建立的Caco-2细胞模型可用于药物吸收机制研究。Objective： To establish the Caco-2 cell model, and to explore the method for applying it in drug research. Methods： Transepithelial electrical resistance （TEER）, the permeability of fluores- cein and the expression of alkaline phosphatase （APK） were used to evaluate Caco-2 cell model. MTT method was used to detect the toxicity of HBSS buffer to Caco-2 cell on the condition of different pH, different temperature and cultivation time. Results： Test results of all the indexes met the require- ments, which indicated that integrity, consistency and permeability of the established Caco-2 cell mod- el were good. The model was not affected when Caco-2 cell was used in drug absorption study within 4 hours. Conclusions： The established Caco-2 cell model can be used to study the absorption mechanism of drugs.

关 键 词：结肠 腺癌 细胞 培养的 碱性磷酸酶 毒性试验 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]