精子DNA损伤、核蛋白组型转换与顶体酶活性及精液参数的相关性分析  

Sperm DNA damage and sperm-nucleoprotein transition correlate to acrosin activity and seminal parameters

作　　者：郑九嘉[1] 杨旭[1] 张李雅[1] 费前进[1] 潘承双[1] 倪吴花[1] 金建远[1] 黄学锋[1] 

机构地区：[1]温州医学院附属第一医院生殖医学中心,浙江温州325000

出　　处：《中华男科学杂志》2012年第10期925-929,共5页National Journal of Andrology

基　　金：温州市科技局资助项目(H20080015)~~

摘　　要：目的:探讨精子DNA损伤、精子核蛋白组型转换与顶体酶活性及精液参数的相关性。方法:收集535例精液标本,采用精子染色质扩散(sperm chromatin dispersion,SCD)检测精子DNA损伤,并与精子核蛋白组型转换、顶体酶活性和WHO手册(第4版)精液参数进行相关性分析。结果:精子DNA损伤与精子核蛋白组型转换、顶体酶活性、精子浓度及前向运动精子这些指标之间比较均具有显著性差异(P<0.01);精子DNA损伤与年龄、核蛋白组型转换、精子浓度和D级精子比例之间呈显著正相关(P<0.01或P<0.05),而与顶体酶活性呈显著负相关(P<0.001),多元逐步回归分析显示年龄、精子浓度、D级精子比例、核蛋白组型转换及顶体酶活性是5个独立的相关变量。精子核蛋白组型转换、顶体酶活性、精子密度和前向运动精子这4个指标的异常率在精子DNA异常组(DFI≥30%)中均显著的高于正常组(DFI<30%)。结论:精子DNA损伤与精子核蛋白组型转换、顶体酶活性及WHO(第4版)精液各参数之间存在密切的联系,可能是评价精子质量的另一项重要的指标。Objective： To investigate the correlation of sperm DNA damage and sperm-nucleoprotein transition with acrosin activity and seminal parameters.Methods： We collected 535 semen samples,assessed sperm DNA damage by sperm chromatin dispersion test,and analyzed the correlation of sperm DNA damage and sperm-nucleoprotein transition with acrosin activity and seminal parameters according to the WHO criteria.Results： Statistically significant differences were observed in sperm DNA damage among sperm-nucleoprotein transition,acrosin activity,sperm concentration and the percentage of grade a ＋ b sperm（P 0.01）.Sperm DNA damage was positively correlated with age,sperm-nucleoprotein transition,sperm concentration and the percentage of grade d sperm（P 0.01 or P 0.05）,but negatively correlated with acrosin activity（P 0.001）.Stepwise linear regression analysis demonstrated that age,sperm concentration,the percentage of grade d sperm,sperm-nucleoprotein transition and acrosin activity were independent variables related to the DNA fragmentation index（DFI）.The abnormality rates of sperm-nucleoprotein transition,acrosin activity,sperm concentration and graded a ＋ b sperm were significantly higher in the sperm DNA damage group（DFI ≥ 30%） than in the normal control（DFI 30%）（P 0.01）.Conclusion： Sperm DNA damage is closely related with sperm-nucleoprotein transition,acrosin activity and seminal parameters,which may become another important independent parameter for the evaluation of sperm quality.

关 键 词：顶体酶 核蛋白组型转换 DNA损伤 

分 类 号：R698.2[医药卫生—泌尿科学]