竞争抑制ELISA法检测霍乱灭活疫苗中脂多糖的含量  

Determination of lipopolysaccharide content in inactivated cholera vaccine by competitive inhibition ELISA

作　　者：陈翠萍[1] 董思国[1] 朱卫华 

机构地区：[1]中国食品药品检定研究院卫生部生物技术产品检定方法及其标准化重点实验室,北京100050 [2]北京绿竹生物制药有限公司,北京100176

出　　处：《中国生物制品学杂志》2012年第10期1366-1367,1372,共3页Chinese Journal of Biologicals

基　　金：国家科技重大专项(2009ZX10004-804)

摘　　要：目的采用竞争抑制ELISA法检测霍乱灭活疫苗中脂多糖(Lipopolysaccharide,LPS)的含量,以替代小鼠免疫力试验方法。方法用竞争抑制ELISA法检测霍乱灭活疫苗中LPS的含量,并分析与小鼠免疫力试验检测结果的一致性。结果霍乱灭活疫苗中LPS的含量与疫苗保护力呈正相关。结论采用竞争抑制ELISA法检测霍乱灭活疫苗中LPS的含量,能够反映疫苗的保护力,为进一步替代小鼠免疫力试验方法奠定了基础。Objective To determine the lipopolysacchardie （LPS） Content in inactivated cholera vaccine by competitive inhibition ELISA instead of immunity test in mice. Methods The LPS content in inactivated cholera vaccine by competitive inhibition ELISA, of which the result was compared with that by immunity test in mice. Results The LPS content in inactivated cholera vaccine was positively related to the immunity in mice. Conclusion Determination of LIPS content in inactivated cholera vaccine by competitive inhibition ELISA reflected the protection of the vaccine, which lald a foundation of further application of the method instead of immunity test in mice.

关 键 词：竞争抑制ELISA 免疫力试验 霍乱灭活疫苗 脂多糖 

分 类 号：R392.3-3[医药卫生—免疫学]