中性粒细胞明胶酶相关脂质运载蛋白对大鼠肾脏缺血再灌注损伤肾小管上皮细胞的保护作用  

Protection of NGAL on renal tubular epithelial cells in rat renal ischemia reperfusion injury

作　　者：臧秀娟[1] 宫丽[2] 洪海娟[1] 姜燕[1] 郑峰[1] 刘梅[1] 于青[2] 

机构地区：[1]上海市松江区中心医院(上海交通大学附属第一人民医院松江分院)肾内科,201600 [2]上海交通大学附属第一人民医院肾内科

出　　处：《中华肾脏病杂志》2012年第10期804-807,共4页Chinese Journal of Nephrology

基　　金：基金项目：上海市卫生局局级科研课题（20114331）;上海市松江区卫生局医学领先合作项目（2011）

摘　　要：目的探讨中性粒细胞明胶酶相关脂质运载蛋白（NGAL）对大鼠缺血再灌注损伤。肾脏肾小管上皮细胞凋亡的保护作用及机制。方法建立大鼠肾脏缺血再灌注模型，雄性SD大鼠随机分为对照组、缺血再灌注模型组、NGAL组；HE染色观察3组大鼠肾组织病理变化；TUNEL法检测肾小管上皮细胞凋亡；实时定量PCR、Western印迹法检测凋亡蛋白fas、bcl－2的表达变化。结果与缺血再灌注模型组比较，NGAL组肾小管上皮细胞凋亡数量显著减少[（8．6＋3．4）／HP比（20．8±3．7）／HP，P〈0．05]；NGAL组肾组织fasmRNA（2．34＋0．51比6．84±2．34，P〈0．05）、fas蛋白（0．65±0．05比0．95±0．08，P〈0．05）表达显著下调，bcl-2蛋白（0．33±0．05比0．24±0．03，P〈0．05）表达显著上调，但bcl－2mRNA表达无明显改变。结论NGAL对大鼠缺血再灌注损伤肾小管上皮细胞有保护作用，其作用可能与减少细胞凋亡、改变凋亡蛋白的表达有关。Objective To investigate the effects of neutrophil gelatinase associated !ipocalin（NGAL） on renal tubular epithelial cells apoptosis and apoptosis-regulated protein fas, bcl-2 in rat renal ischemia reperfusion injury （IRI）. Methods Renal IRI models of rats were established. Rats were randomly divided into 3 groups, including control group, IRI model group and NGAL group. The pathological change of kidney tissue was investigated by hemotoxylin-eosin staining. Renal tubular epithelial cells apoptosis was detected by TUNEL method. Expression of fas and bcl-2 was measured by real-time PCR and Western blotting. Results Compared with IRI model group, NGAL group showed a decreased number of renal tubular epithelial cells apoptosis [（8.6±3.4）/HP vs （20.8±3.7）/HP, P〈0.05], down-regulated fas mRNA （2.34±0.51 vs 6.84±2.34, P〈 0.05）, fas protein （0.65±0.05 vs 0.95±0.08, P〈0.05） and up-regulated bcl-2 protein （0.33± 0.05 vs 0.24±0.03, P〈0.05）, but the bcl-2 mRNA had no significant change. Conclusion NGAL can protect renal tubular epithelial cells in renal IRI, which may be associated withdecreasing cell apoptosis and adjusting protein expression by apoptosis-regulated cytokines.

关 键 词：再灌注损伤 细胞凋亡 肾小管上皮细胞 凋亡蛋白 中性粒细胞 明胶酶相关脂质运载蛋白 

分 类 号：R692[医药卫生—泌尿科学]