邻苯二甲酸二丁酯对亲代和子代斑马鱼生殖毒性的比较研究  

Reproductive Toxicity of Dibutyl Phthalate in Parent and Progeny Zebrafish

作　　者：徐浩[1] 王玉柱[2] 温成丽[2] 成倩倩[2] 田芳[2] 夏敏杰[2] 李润生[2] 李卫华[2] 丁训诚[2] 

机构地区：[1]上海中医药大学附属龙华医院,200032上海 [2]上海市计划生育科学研究所,200032上海

出　　处：《实验动物与比较医学》2012年第5期383-389,共7页Laboratory Animal and Comparative Medicine

摘　　要：目的探讨邻苯二甲酸二丁酯（DBP）对亲代（F0）、子代（F1）斑马鱼的生殖毒性。方法将48对成年斑马鱼随机分为4组：空白对照组、溶剂对照组、625唱／LDBP低剂量组和1250rag／LDBP高剂量组。连续染毒DBP30d后，将各组F0代斑马鱼交配，记录产卵数、受精率以及72h孵化率。将F0和F1代斑马鱼在清水中饲养180d，进行F1代斑马鱼交配试验，且将F1代DBP高剂量组与对照组斑马鱼进行置换交配，记录产卵数、受精率及孵化率。进行F0代和F1代斑马鱼性腺组织病理学检查，并测定卵黄蛋白原（VTG）mRNA的表达水平。结果与对照组和DBP低剂量组相比，DBP高剂量组F0代和F1代斑马鱼的生殖能力显著下降；置换交配实验显示DBP对雄性斑马鱼的生殖能力影响更为显著。组织病理学检查显示F0代雄鱼精巢中精子数量减少，间质细胞稍有增多，精母细胞增多。而F1代雄鱼精巢发育受到明显抑制，出现生精小管畸形，精子数量明显减少，以及间质细胞增生。RT—PCR结果显示DBP各剂量组F0代和F1代雄性斑马鱼VTG基因无明显表达，提示DBP对斑马鱼无雌激素样效应。结论DBP高剂量组染毒可影响F0代和F1代斑马鱼的正常发育，使产卵量减少，受精率显著下降。F0代和F1代雄性斑马鱼生精小管畸形、精巢中精子数量减少与间质细胞增生同时存在。Objective To study the reproductive toxicity effects of dibutyl phthalate （DBP） on F0 （parent） and F1 （parent） zebrafish. Methods Fourty-eight pairs of adult zebrafish were randomly divided into 4 groups： blank control group, 0.01% acetone solvent control group, 625 mg/L DBP low-dose group, and 1250 rag/L DBP high-dose group. After 30-day exposure, F0 zebrafish in each group were mated and the number of eggs, fertilization rate and 72-hours hatchability were recorded. After being kept for 180 days in clean water, F0 and F1 zebrafish in each group were again mated, and the replacement mating test were conducted. The Vetellogenin （VTG） mRNA of F0 and F1 zebrafish was measured by RT-PCT, and gonad histopathology was performed. Results The fertilization of F0 and F1 zebrafish in 1250 mg/L DBP group was significantly lower than that in blank control or in 625 mg/L DBP group; histological examination showed that DBP slightly inhibited ovarian development of the Fo and F1 zebrafish and slightly increased the cortical vesicular stage oocyte （Coc） and perinucleolar stage oocyte （Poc）. In F0 male zebrafish of 1250 mg/L DBP group, the sperm count in the testes was reduced, and the spermatocyte （sc） and Leydig cells were slightly increased. In F1 male zebrafish of 1250 mg/L DBP group, the development of testes was inhibited significantly, manifested as the co-existence of abnormal seminiferous tubules, reduced sperm count and Leydig cell hyperplasia. Exposure to DBP could not induce the expression of VTG mRNA in either F0 or F1 male zebrafish, indicating that DBP has no estrogen-like effects on zebrafish. Conclusion Exposure to 1250 mg/L DBP can induce reproductive toxicity in F0 and F1 zebrafish. The number of eggs and egg hatchability were reduced. Abnormal seminiferous tubules, reduced sperm count as well as Leydig cell hyperplasia were found.

关 键 词：斑马鱼 邻苯二甲酸二丁酯(DBP) 生殖毒性 Fo FL 

分 类 号：R114[医药卫生—卫生毒理学]