抗树鼩、灰仓鼠和长爪沙鼠IgG抗体的制备及标记  

Preparation and Labeling of Anti-IgG in Tupaia belangeri, Cricetulus migratorius and Merionesun guiculatus

作　　者：付瑞[1] 李晓波[1] 王吉[1] 卫礼[1] 王淑菁[1] 岳秉飞[1] 贺争鸣[1] 

机构地区：[1]中国食品药品检定研究院实验动物资源研究所,北京100050

出　　处：《实验动物与比较医学》2012年第5期441-445,共5页Laboratory Animal and Comparative Medicine

基　　金：国家科技支撑计划.2009BAI83B02

摘　　要：目的制备抗树鼩、长爪沙鼠和灰仓鼠IgG抗体，并分别用异硫氰酸荧光素和辣根过氧化物酶进行标记。方法采用ProteinG分别提纯树鼩、长爪沙鼠和灰仓鼠血清中的IgG，纯化后免疫家兔，制备兔抗三种动物IgG抗体；采用亲和层析纯化兔抗三种动物IgG抗体，并分别进行FITC和HRP标记。结果分别获得抗灰仓鼠、树鼩和长爪沙鼠IgG抗体，浓度分别为：2．2mg／ml、2．3mg／ml和2．3mg／ml；FITC标记的兔抗灰仓鼠、树鼩和长爪沙鼠IgG含量分别为：1．19mg／ml，1．36mg／ml和1．55mg／ml；HRP标记的兔抗灰仓鼠、树鼩和长爪沙鼠IgG含量分别为：0．84mg／ml、1．09mg／ml和1．31mg／ml。结论得到了适合检测用的FITC和HRP标记二抗，为建立这三种动物致病微生物和寄生虫检测方法提供了试剂。Objective To prepare anti-IgG of Tupaia belangeri, Cricetulus migratorius and Merionesun guiculatus, and label them by using FITC and HRP. Method The IgG of these three animals were purified using Protein G and the anti-IgG were obtained from immunized rabbits. The anti-IgG were purified through affinity chromatography and labeled by FITC and HRP. Results The anti-IgG concentration of Cricetulus migratorius, Tupaia belangeri and Merionesun guiculatus was 2.2 mg/ml, 2.3 mg/ml and 2.3 mg/ml respectively. The IgG content of FITC labeled anti-IgG for each animal was 1.19 mg/ml, 1.36 mg/ml and 1.55 mg/ml respectively. The IgG content of HRP labeled anti-IgG for each animal was 0.84 mg/ml, 1.09 mg/ml and 1.31mg/ml respectively. Conclusion The study successfully obtained the FITC and HRP labeled anti-IgG, which can be used for the establishment of microorganism detection in these three animals.

关 键 词：树嗣 长爪沙鼠 灰仓鼠 IGG 标记 

分 类 号：Q95-33[生物学—动物学]