着色性干皮病A基因表达对A549／DDP化疗敏感性的影响  

Effect of XPA expression on the chemotherapy sensitivity of A549/DDP cells

作　　者：张强[1,2] 吴金香[1] 魏玉平[3] 郝俊青[4] 黄山英[5] 董亮[1] 

机构地区：[1]山东大学齐鲁医院呼吸科,山东济南250012 [2]济南市第三人民医院,山东济南250101 [3]山东大学医学院生物化学与分子生物学研究所,山东济南250012 [4]山东省高唐县人民医院,山东聊城252800 [5]山东大学教育部,卫生部心血管重构与功能研究重点实验室,山东济南250012

出　　处：《细胞与分子免疫学杂志》2012年第11期1182-1184,1187,共4页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的:探讨沉默着色性干皮病A(XPA)基因表达在非小细胞肺癌耐药细胞株顺铂化疗敏感性的影响。方法:采用免疫组化法、实时定量PCR(qPCR)及Western blot方法检测非小细胞肺癌患者肿瘤组织中XPA的表达情况。应用qPCR及Western blot方法检测A549/DDP细胞经XPA-shRNA转染后XPA-mRNA及其蛋白表达。通过MTT法检测沉默XPA基因后A549/DDP细胞凋亡情况及其对顺铂的敏感性。结果:肺癌组织XPA表达水平明显高于癌旁组织;沉默XPA基因能够促进A549/DDP细胞凋亡,并能提高A549/DDP对顺铂的药物敏感性。结论:沉默XPA基因表达能够逆转肺癌A549/DDP细胞对顺铂的耐药性。AIM： TO investigate the influence on platinum- based chemotherapy sensitivity by silencing xeroderma pigmentosum group A （XPA） gene expression in non-small cell lung cancer （NSCLC） drug resistance cell lines （A549/ DDP）. METHODS： We detected the expression of XPA in lung normal and tumor tissues by immunohistochemistry, quantitative real-time PCR （qPCR） and Western blotting. We silenced XPA expression in A549/DDP cells by XPA- shRNA transfection, and detected the expression of XPA by qPCR and Western blotting. The cell sensitivity to cisplatin and the apoptosis of A549/DDP cells transfected with XPA- shRNA were determined by MI-F assay. RESULTS. The expression of XPA was higher in NSCLC tissues than that in normal lung tissues. Silencing XPA gene increased the apoptosis and sensitivity of A549/DDP cells to cisplatin. CONCLUSION： Silencing XPA gene can partly reverse the cisplatin resistance in human cisplatin-resistant NSCLC cell line A549/DDP.

关 键 词：非小细胞肺癌 XPA A549 DDP细胞 顺铂耐药 

分 类 号：R392-33[医药卫生—免疫学] R734.2[医药卫生—基础医学]