多次强脉冲光照射对皮肤成纤维细胞老化指标的影响  

Effect of multiple IPL treatment on cell senescence markers in skin fibrublasts

作　　者：王瑞艳[1] 刘玮[1] 赵小忠[1] 袁小英[1] 刘红梅[1] 田燕[1] 

机构地区：[1]空军总医院皮肤科,北京100142

出　　处：《中华医学美学美容杂志》2012年第5期364-367,共4页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：国家自然科学基金资助项目（编号：30872269）

摘　　要：目的探讨多次强脉冲光（intense pulsed light．IPL）照射对皮肤成纤维细胞老化指标的影响，并用长波紫外线（ultraviolet A，UVA）作对比，观察多次强脉冲光照射能否造成细胞老化。方法实验分3组．一组未接受照射作为对照组，其他两组分别接受UVA9J／cm^2和IPL15J／cm^2照射。每天1次，连续照射5d。在第6天，收集细胞，分别进行p半乳糖舒酶（SA-β-Gal）染色及细胞周期、细胞内活性氧和端粒K度的测定。结果连续5dIPL照射后．SA-β-Gal染色和端粒长度与正常对照组相比，未见明显变化（P〉0．05）。细胞周期中G1期所占百分比下降。细胞内活性氧下降（P〈0．05）；连续jdUVA照射后，与正常对照组相比，SA-β-Gal染色阳性细胞的百分数增加，细胞周期中G1期所占百分比未见明显变化，细胞内活性氧上升，端粒长度缩短（P〈0．05）。结论多次UVA照射可诱导细胞老化，但多次IPL照射不会出现细胞老化。Objective To study the effect of multiple IPL treatment on cell senescence markers of skin fibroblasts using UVA as a control and to make clear whether the multiple 1PL treatment may resuh in cell senescence. Methods Cells were divided into three groups： one group without irradiation as a control, one group receiving IPI. treatment with 15 J per cme , and the last group receiving UVA irradiation with 9 J per cme. IPI. and UVA irradiation were performed once a day during five days. On the sixth day, the cells were collected. Senescencc-associated β-galactosidase （SA-/3-Gal） staining, cell cycle, reactive oxygen species （ROS） and telomere length were determined. Results Our results showed that five consecutive days of IPI. irradiation had no effect on the activity of SA-β-Gal and te- lomere length and decreased the G1% of cell cycle and the level of ROS in comparison with the control group （P〈0.05）. On the contrary, five consecutive days of UVA irradiation increased the activity of SA-β-Gal and the level of ROS. shortened the length of telomere and no obvious change in the G1 % of cell cycle in comparison with the control group. Conclusions Muhiple UVA irradiations induce cell senescence. On the contrary, multiple IPL treatments could not induce cell senescence.

关 键 词：强脉冲光 β半乳糖苷酶 细胞周期 细胞内活性氧 端粒长度 长波紫外线 

分 类 号：R758.14[医药卫生—皮肤病学与性病学]