恶臭假单胞菌TS1138透性化细胞的制备及其转化DL-ATC生成L-半胱氨酸的特性研究  

Preparation of Permeabilized Pseudomonas Putida TS 1138 Cells and Its Properties of Converting DL-ATC to L-cysteine

作　　者：陈晓云[1] 杜军 高智慧[3] 姚瑞娟[1] 张奇[1] 

机构地区：[1]南开大学药学院,天津300071 [2]中国生物发酵产业协会,北京100833 [3]南开大学生命科学学院,天津300071

出　　处：《南开大学学报（自然科学版）》2012年第4期99-104,共6页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：天津市科技支撑计划(09ZCKFSH00900)

摘　　要：探讨了恶臭假单胞菌TS1138菌体透性化细胞的制备工艺,并对所得透性化细胞转化DL-ATC生成L-半胱氨酸的反应特性进行了研究.结果表明,以2%的甲苯为渗透剂,在25℃条件下对浓度为20%的菌悬液渗透处理30 min,所得透性化细胞的酶活达1 475 U/g,为未渗透细胞的10倍.该透性化细胞的最适转化反应温度为35℃,最适反应pH为8.0,在pH 7.5～9.0范围内稳定,Cu2+、Ag+和Zn2+对透性化细胞的转化反应具有较强抑制作用,Cu2+的抑制作用最强.The permeabilization of Pseudomonas putida TS1138 cells and the properties of perme- abilized cells involved in the conversion of DL-ATC to L-cysteine were studied. A proper process was achieved with 20% of wet cell suspension permeabilized by 2% of toluene for 30 rain at 25 ~C. The activity of permeabilized cells reached 1 475 U/g which was 10 times as much as that of rest cells. The permeahilized cells exhibited optimal conversion activity at 35 ℃and pH 8.0, and were stable from pH 7.5 to 8.0. Its conversion activity was inhibited strongly by Cu2＋ and moderately by Ag ＋ and Zn2 ＋.

关 键 词：恶臭假单胞菌 DL-ATC L-半胱氨酸 透性化细胞 

分 类 号：Q936[生物学—微生物学]