大庆油田聚驱后油藏内源微生物激活剂的筛选和效果评价  

Screening and Effect Evaluating the Activator for Indigenous Microbe of Daqing Flowing Gel Flood Reservoirs

作　　者：伍晓林[1,2] 赵玲侠[3] 马挺[3] 俞理[4] 乐建君[2] 

机构地区：[1]北京大学工学院,北京100083 [2]大庆油田有限责任公司勘探开发研究院,黑龙江大庆163712 [3]南开大学生命科学学院分子微生物与技术教育部重点实验室,天津300071 [4]中国石油天然气股份有限公司勘探开发研究院廊坊分院,河北廊坊065000

出　　处：《南开大学学报（自然科学版）》2012年第4期105-111,共7页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家“863”计划(2009AA063502);国家自然科学基金(50804024);中石油科技重点项目(2008A-1403)

摘　　要：聚合物驱油后的油藏含水大幅回升,操作成本高,传统化学方法很难继续提高采收率,而内源微生物采油技术是最有希望的接替技术之一.针对大庆油田聚驱后油藏,利用分子生态学技术考察目标油藏的菌群结构,并通过统计学方法对内源微生物激活剂进行筛选和效果评价.结果表明目标油藏具有多种采油功能微生物,确定以糖蜜,硝酸盐,磷酸盐及酵母粉为主的激活剂体系,该体系能显著提高内源微生物的数量,有益菌浓度提高1～3个数量级,代谢产生的表面活性剂能将原油完全乳化分散,形成稳定的悬浊液,同时也能兼顾厌氧微生物的生长及产甲烷菌代谢产甲烷,因此具备实施内源微生物采油的潜力.The water content rise again in flowing gel flood reservoirs, and because of the high cost, it may not continue enhancing oil recovery by traditional chemical method. Microbe enhancing oil recovery （MEOR） technology is one of the potential competences. This study focused on screening and evaluating the activator of Daqing flowing gel flooding reservoirs. The results of flora analysis showed that there are a plenty of flooding-functional microbes in the targeted reservoir. The activator system, which contain molasses, nitrate, phosphate, yeast powder, can remarkably achieve indigenous microbe growth. The crude oil can be entirely emulsified by metabolic product of the indigenous microbe, which can also produce methane gas under anaerobic conditions. It seemed by all certifications that the activator had the potential application in MEOR.

关 键 词：聚驱后油藏 内源微生物 激活剂 筛选 评价 

分 类 号：Q93[生物学—微生物学]