大豆TCP转录因子家族结构域分析及功能预测  

Domain Analysis and Function Prediction of TCP Transcription Factors Family in Soybean

作　　者：刘洋[1,2] 张慧[1,2] 辛大伟[1,2] 王琳琳[1,2] 张丽伟[1,2] 刘春燕[2] 陈庆山[1,2] 胡国华[1,2] 

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030 [2]黑龙江省农垦科研育种中心,黑龙江哈尔滨150090

出　　处：《大豆科学》2012年第5期707-713,717,共8页Soybean Science

基　　金：抗除草剂转基因大豆新品种培育项目(2011ZX08004-001-06);现代农业产业体系资助项目(CARS-04-02A);黑龙江省普通高等学校新世纪优秀人才培养计划(1252-NCET-004)

摘　　要：利用已知的拟南芥TCP基因和大豆基因组数据库,通过生物信息学方法,鉴定并获得了大豆TCP家族基因序列,基因定位信息。共鉴定大豆TCP基因54个,分布在大豆除14号染色体外的其他19条染色体上。对54个基因进行进化和聚类分析及功能结构域的分析,发现全部具有高度保守的TCP结构域。根据结构域差异和系统发育分析的结果,将大豆TCP转录因子家族分成2个TCP亚家族。以生物信息学手段和已有的其他物种的TCP基因进行了比较分析,大豆TCP基因占总数的3.5%,与拟南芥同源基因比较,序列长度存在相似性。对启动子元件分析显示,TCP基因含有大量与在子叶、根、茎处大量表达有关的元件及对分生组织有作用的元件。TCP transcription factors gene sequences were identified based on soybean genome database and Arabidopsis TCP genes by the bioinformatics method.Fifty-four soybean TCP genes all contained highly conservative TCP structure domain,were found and distributed in 19 chromosomes except chromosome No.14.They could be classified into 2 sub-families according to their structure and phylogeny relationship.By compare to TCP genes of other species,the evolution structure was also be analyzed.Soybean TCP genes account for 3.5% of the total amount,and compared with Arabidopsis homologous genes,the sequence length exist similarities.Analysis of promoter elements showed that the TCP gene contains elements,they refer to expression in the cotyledons,roots,stems and has the function of meristem tissue.All results provide a basis for the further study of the soybean TCP transcription factor.

关 键 词：大豆 TCP转录因子 结构域分析 功能预测 

分 类 号：S565.1[农业科学—作物学]