大豆酰基载体蛋白硫酯酶基因及其启动子表达方式的初步分析  

Preliminary Analysis of the Expression Pattern of Soybean acyl-ACP Thioesterase Gene and Promoter

作　　者：赵艳[1] 杨晓杰[1] 范震宇[1] 祁宏英[1] 

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006

出　　处：《大豆科学》2012年第5期714-717,共4页Soybean Science

基　　金：齐齐哈尔大学青年教师科研启动支持计划项目(2010K-M09);黑龙江省教育厅科学技术研究项目(12521611);作物生物学国家重点实验室开放课题(2012KF13);国家自然科学基金(40971053)

摘　　要：利用实时定量PCR方法,检测大豆酰基载体蛋白硫酯酶(acyl-ACP thioesterase)基因在大豆各组织中的表达方式,结果显示该基因在大豆根、茎、叶、花中的表达活性低,而种子中的表达活性较高。利用PCR方法,克隆大豆acyl-ACP thioesterase基因5'端上游2 057 bp序列,命名为AP。在线启动子预测软件分析结果表明AP序列中含有多种典型的种子特异表达元件,如RY repeat、SEF1 motif、SEF3 motif、SEF4 motif、E-box、ACGT等顺式作用元件,推测大豆acyl-ACP thioesterase基因启动子具有种子特异表达活性。The expression pattern of soybean acyl-ACP thioesterase gene in soybean tissues was detected by real-time quantitative PCR （RTQ-PCR）. The result showed that there were a little activity in roots, stems,leaves and flowers, but there was higher activity in seeds. The 5＇-flanking upstream sequence of soybean acyl-ACP thioesterase gene, named AP, was isolated from soybean genomic DNA by PCR method, and the length was 2 057 bp. Sequence analysis by PLACE revealed that this fragment contained a series of motifs related to seed-specific promoters ,such as RY repeat, SEF1 motif, SEF3 motif, SEF4 motif, E-box and ACGT. It can be inferred that AP promoter possess the function driven downstream gene expression exclusively in soybean seeds.

关 键 词：大豆 酰基载体蛋白硫酯酶基因 启动子 克隆 

分 类 号：S565.1[农业科学—作物学]