大豆再生相关基因GmESR1的克隆及其表达分析  

Cloning and Expression Analysis of Regeneration Related Gene GmESR1 in Soybean

作　　者：刘明[1] 苏安玉[2] 李静[1] 孙晶[1] 王敏[1] 李思楠[1] 李文滨[1] 武小霞[1] 

机构地区：[1]东北农业大学农学院,国家教育部大豆生物学重点实验室,黑龙江哈尔滨150030 [2]东北农业大学资源与环境学院,黑龙江哈尔滨150030

出　　处：《大豆科学》2012年第5期731-733,738,共4页Soybean Science

基　　金：国家自然科学基金(31071438);黑龙江省自然科学基金(201117);黑龙江省教育厅项目(11551048);东北农业大学人才启动基金(2009RC47)

摘　　要：利用同源克隆技术,对拟南芥再生相关基因ESR1进行同源序列比对,得到大豆再生相关基因GmESR1。结果表明,大豆再生相关基因GmESR1全长1 164 bp,转录区为981 bp,编码326个氨基酸,含有一个AP2/ERF结构域,属于AP2家族成员。对大豆叶片进行细胞分裂素处理,实时定量PCR技术分析表明细胞分裂素能明显促进GmESR1基因表达。In order to establish an efficient and stable soybean regeneration system,a soybean regeneration-related gene GmESR1 was cloned through candidate gene homology-based method and analyzed its function.Our results showed that the complete length and coding region of GmESR1 was 1 164 and 981 bp respectively,and encoded a peptides consisting of 326 amino acids.Protein sequence analysis indicated that GmESR1 contains an AP2/ERF domain,and belongs to the AP2 family,which contained members of cytokinin inducible transcription factors that were extensively involved in plant resistance against stress and transcriptional regulation during shoot regeneration.Blast search showed that GmESR1 shared high homology with Arabidopsis ESR1 gene,which was also a member of the AP2 family.Exogenous hormone treatment experiment showed that the expression level of GmESR1 was increased under the cytokinin treatment.

关 键 词：大豆 再生 GmESR1 

分 类 号：S565.1[农业科学—作物学]