转ThIPK2基因大豆的农艺性状及光合生理研究  

Agronomic Traits and Photosynthetic Traits of Transgenic Soybean Overexpressing ThIPK2

作　　者：刘淼[1,2] 李冬梅[1] 王志坤[1] 孟凡立[1] 李永光[1] 武小霞[1] 张洪霞[3] 李文滨[1] 

机构地区：[1]大豆生物学教育部重点实验室,农业部大豆生物学与遗传育种北方区域重点实验室,东北农业大学大豆研究所,黑龙江哈尔滨150030 [2]黑龙江省农业科学院耕作栽培研究所,黑龙江哈尔滨150086 [3]中国科学院上海生命科学研究院植物生理生态研究所,上海200032

出　　处：《大豆科学》2012年第5期775-777,共3页Soybean Science

基　　金：国家转基因重大专项(2011ZX08004-002);农业部大豆产业技术体系项目(CARS-04-PS04)

摘　　要：将抗逆基因ThIPK2转入东农50,得到稳定遗传株系,将T4代株系种子正常播种,将转基因植株与对照东农50的生育期、株高、主茎节数、分枝数、主茎荚数、分枝荚数、单株粒数等性状进行比较,研究ThIPK2基因对大豆农艺性状方面的影响。结果表明,在正常条件下,转基因株系和对照植株相比,生育期并没有改变,而株高、主茎荚数、分枝荚数、分枝数和单株粒数农艺性状呈显著或极显著提高,这表明ThIPK2基因对提高大豆产量有利。干旱胁迫导致大豆叶片各项光合生理指标下降;但转ThIPK2基因大豆的各项光合生理指标高于非转基因对照。经干旱胁迫后,转基因植株与对照相比积累了更多的干物质。Dongnong50 was transformed with ThIPK2, a stress-tolerance gene,and transgenic progenies with ThIPK2 with sta- ble heredity characters were obtained in this study. The seeds of T4 generation were planted on transgenic experimental base, investigated agronomic traits and compared to control plants DongnongS0. For the transgenic plants, the growth duration re- mained stable, while significant increase were observed for plant height, pods on main stem, pods on branch, and seeds per plant. Hence, the ThlPK2 gene is beneficial to improve soybean yields. Drought stress declined all indicators of photosynthetic physiology. But indicators of transgenic soybean were higher than non-transgenic soybean under drought stress. In addition, transgenic plants accumulated more dry matter than controls under drought condition.

关 键 词：ThIPK2 转基因大豆 抗逆 生育期 农艺性状 

分 类 号：S565.1[农业科学—作物学]