尿素对大豆胞囊线虫的抑制作用  

Suppression of Urea on Soybean Cyst Nematode

作　　者：宋洁[1,2] 许艳丽[1] 姚钦[1,2] 李慎鹏[3] 季丹丹[3] 

机构地区：[1]中国科学院东北地理与农业生态研究所黑土区农业生态院重点实验室,黑龙江哈尔滨150081 [2]东北林业大学生命科学学院,黑龙江哈尔滨150040 [3]黑龙江大学农学院,黑龙江哈尔滨150080

出　　处：《大豆科学》2012年第5期784-788,共5页Soybean Science

基　　金：国家自然科学基金项目(30971900);黑龙江省自然科学基金项目(C200630)

摘　　要：采用实验室生测和温室盆栽的方法,研究了尿素对大豆胞囊线虫卵孵化、二龄幼虫活性的抑制作用以及对大豆胞囊线虫的控制效果。结果显示,尿素的浓度在0.03～1.00 mol·L-1范围内,对大豆胞囊线虫的卵孵化和二龄幼虫(J2)均具有抑制作用,且随着浓度的升高抑制作用增强,当尿素浓度到达1.0 mol·L-1时,卵孵化的抑制率最高达到96%,J2的致死率可达到100%。温室盆栽结果显示,施用尿素后,土中和大豆根部的大豆胞囊线虫种群密度显著降低,且大豆株高、地上鲜重和地下鲜重等均有所增加,其中鲜重总增重率可达42.8%。研究结果证明尿素对大豆胞囊线虫具有明显的致死作用,且在一定程度上能控制该病害的发生。A study was conducted in lab and greenhouse to determine the effect of the urea on eggs,second-stage juveniles（J2）of Heterodera glycines and control efficiency of Soybean Cyst Nematode（SCN）.The results showed that the urea inhibited the nematode egg hatch and the activity of J2 at the concentration of urea from 0.03 to 1.0 mol·L-1,and with the increase of concentration,the suppressive effect rose accordingly.The suppressive rate of egg hatching and mortality of J2 reached 96% and 100% when urea concentration was at 1.0 mol·L-1,respectively.In greenhouse,application of urea significantly reduced the population of SCN in soil and soybean root.The application of urea also enhanced height,fresh and dry weight of shoots and roots of soybean,and the increase rate of fresh weight was 42.8%.The urea has lethal effect on SCN,and the population of SCN is suppressed by urea in a certain extent.

关 键 词：大豆胞囊线虫 尿素 卵孵化 二龄幼虫 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]