空心莲子草有效部位提取物抗甲型H3N2流感病毒作用  

Antiviral activity of Alternanthera Philoxeroides effective fractions extracts against virus A H3N2

作　　者：李爽[1] 彭晓芝[1] 廖鹏程[1] 陈树和[1] 刘焱文[1] 

机构地区：[1]湖北中医药大学中药资源与中药化学省级重点实验室,武汉430061

出　　处：《天津中医药》2012年第5期478-480,共3页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30470194)

摘　　要：[目的]以空心莲子草有效部位提取物为研究对象,进一步验证其抗甲型H3N2流感病毒的生物活性,为其开发应用提供依据。[方法]以利巴韦林注射液作为空心莲子草有效部位提取物抗病毒实验的阳性对照药物,观察甲型H3N2流感病毒感染幼犬肾(MDCK)细胞后引起的细胞病变效应(CPE),通过四甲基偶氮唑蓝(MTT)法检测细胞活性,采用治疗指数(TI)作为药物抗病毒效果的评价指标,评价空心莲子草有效部位提取物抗甲型H3N2流感病毒的生物活性。[结果]空心莲子草有效部位提取物对流感病毒有明显抗病毒生物合成作用,其TI为(2.43±0.01),但其阻止流感病毒吸附与直接杀伤流感病毒的作用不明显。[结论]空心莲子草有效部位提取物有较强的抗流感病毒作用,其作用方式为抗病毒生物合成,且呈剂量依赖性。[Objective] Using Ahernanthera Philoxeroides effective fractions extracts as a research object, to further validate its biological activity in vitro anti-influenza A H3N2 influenza virus, in order to provide a basis for its development and application. [Methods] Ribavirin injection was used as a positive control drug. By observing the cytopathic effect （CPE） caused by the influenza A H3N2 influenza virus infection in MDCK ceils, using MTr method to check the cell activity, and using therapeutic index （TI） as an evaluation of antiviral effect in vitro to evaluate the antiviral activity of the drugs. To evaluate the biological activity against influenza A H3N2 influenza virus of Ahemanthera Philoxeroides effective fractions extracts. [Results] Alteruanthera Philoxeroides effective fractions of extracts had significant antiviral biosynthesis of influenza virus. Its therapeutic index （TI） were （2.43±0.01）. But its preventing the influenza virus adsorption and direct anti-influenza virus was not obvious. [Conclusion] Ahernanthera Philoxeroides effective fractions extracts have certain antiinfluenza virus, and its form is antiviral biosynthesis of influenza virus, and has a dose-dependent.

关 键 词：空心莲子草 MDCK细胞 甲型H3N2流感病毒 抗病毒作用 

分 类 号：R285.5[医药卫生—中药学]