同种反应性自然杀伤细胞对小鼠单倍体相合骨髓移植后免疫重建的影响  

Effect of Alloreactive Natural Killer Cells on Immune Reconstitution in Mouse Haploidentical Bone Marrow Transplantation

作　　者：王华[1] 王慧[1] 刘英慧[1] 冯四洲[2] 韩明哲[2] 

机构地区：[1]烟台毓璜顶医院血液科,山东烟台264000 [2]中国医学科学院北京协和医学院血液病医院、血液学研究所,天津300020

出　　处：《中国实验血液学杂志》2012年第5期1171-1175,共5页Journal of Experimental Hematology

基　　金：山东省自然科学基金资助项目(编号ZR2010HQ014);烟台市科学技术发展计划资助项目(编号2010148-9)

摘　　要：本研究旨在探讨同种反应性自然杀伤(alloNK)细胞对小鼠单倍体相合骨髓移植(BMT)后免疫重建的影响。以(C57BL/6×BALB/c)BCF1(H-2d/b)为供鼠,BALB/c(H-2d)为受鼠,建立小鼠单倍体相合BMT模型,根据回输物不同将实验分为单纯BMT组、非同种反应性NK(non-alloNK)细胞组、alloNK细胞组。移植后2个月以胸腺组织病理、脾脏NK细胞比例、脾细胞增殖反应及脾细胞培养上清中细胞因子浓度等指标评价alloNK细胞对免疫重建的影响。结果显示,光学显微镜下各组小鼠胸腺组织病理学表现无明显差别;移植后2个月non-alloNK组小鼠脾脏NK细胞比例显著低于单纯BMT组(P<0.05);各组小鼠脾细胞体外增殖反应无明显差别;alloNK组小鼠脾细胞培养24和48 h时培养上清中干扰素-γ的浓度均显著低于其他2组(P<0.05),而各组之间白介素4水平无明显差别。结论:alloNK细胞用于单倍体BMT小鼠不损伤胸腺的结构,可能诱导Th2免疫反应的偏移。The study was purposed to investigate the effect of alloreactive natural killer（alloNK）cells on immune reconstitution in murine haploidentical bone marrow transplantation（BMT）.The murine model of haploidentical BMT was established by using（C57BL/6×BALB/c）BCF1（H-2d/b）mouse as the donor,and BALB/c（H-2d） mouse as the recipient.Recipient mice were divided into BMT group,nonalloreactive NK（non-alloNK） cell group and alloNK cell group according to different transfusion.The effect of adding alloNK cells to transfusion was assessed by thymus pathology,the proportion of spleen NK cells,the spleen cell proliferation,the IFN-γ and IL-4 concentrations producted at 24 and 48 h of recipient spleen cell culture supernatant at 2 months after BMT.The results showed that there were no obvious difference in thymus tissue among 3 groups under the optical microscope.The proportion of recipient spleen NK cells in non-alloNK group was significantly lower than that in BMT group（P0.05）.There was no significant difference in proliferation of the recipient spleen cells among 3 groups at 2 months after BMT.The IFN-γ concentration producted at 24 and 48 h of recipient spleen cell culture supernatant in alloNK group was significantly lower than that in other 2 groups at 2 months after BMT（P0.05）.The IL-4 concentration in each group was not significantly different（P0.05）.It is concluded that alloNK cells do not damage the thymus structure and may induce Th2 immune response in murine haploidentical BMT.

关 键 词：同种反应性NK细胞 单倍体骨髓移植 免疫重建 

分 类 号：R457.7[医药卫生—治疗学]