人胎盘绒毛组织悬液支持脐血CD34^+细胞体外扩增的作用  

Effect of Human Placental Extract on Proliferation of Human Umbilical Cord Blood CD34^+ Cells In Vitro

作　　者：马坤[1] 姚慧[1] 张敏 郭晶晶[3] 程琳[1] 李嘉惠[1] 刘志杰[1] 

机构地区：[1]山东大学附属千佛山医院儿科,山东济南250014 [2]山东大学附属千佛山医院产科,山东济南250014 [3]山东大学附属济南市中心医院内分泌科,山东济南250014

出　　处：《中国实验血液学杂志》2012年第5期1183-1186,共4页Journal of Experimental Hematology

摘　　要：本研究旨在探讨不同时期的胎盘组织悬液对脐血造血干细胞体外扩增的作用,进一步了解随着胎龄的增长胎盘造血功能发生的变化。应用早期(B组)、中期(C组)和足月(D组)胎盘绒毛组织悬液,并设定空白对照(A组),以共培养的方式分4组进行体外扩增脐血造血干细胞并培养观察扩增后的集落形成能力。结果表明,以接种初浓度为对比,共培养7 d后A组细胞呈逐渐衰减趋势,B组扩增效果不明显,仅为扩增前的1.40±0.20倍,C组和D组造血干细胞均能有效扩增,扩增倍数分别为2.93±0.50和4.80±0.40。集落培养14 d后的结果显示,C、D组集落形成总数明显高于A、B组,C组CFU-GM、CFU-GEMM、BFU-E集落数均略高于D组。结论:在没有外源性细胞因子存在的情况下,不同时期的胎盘绒毛组织悬液均可以支持造血干细胞的体外扩增,且随着胎盘发育其支持造血的功能呈不断增强的趋势。扩增后的造血干细胞的集落形成能力却呈先增强后减弱的现象,中期胎盘培养扩增后的干细胞集落形成能力最强,略强于足月期胎盘扩增后的造血干细胞。The study was aimed to investigate the hematopoietic function of placenta tissue and clarify the effect of human placental chorionic tissue in different periods on proliferation of hematopoietic stem cells in vitro,in order to further understand the changes of the hematopoietic function of placenta with the time prolonging.The experiments were divided into four groups： early placenta（group B）,mid-term placenta（group C）,full-term placenta（group D）,and blank group（group A）.The hematopoietic stem cells were amplified in co-culture way,and the colony formation ability after the expansion was observed.The results showed that compared to initial concentration,the CD34＋ cells cultured with full-term placenta were amplified by（2.60±0.20） times,which was significantly higher than those CD34＋ cells cultured with mid-term placenta（1.74±0.24） and early placenta（1.14±0.12）,but that in blank group was reduced without amplification.After culture for 14 days,the colony number of group C and group D were significantly higher than that of group A and group B.Among them the number of CFU-GM,CFU-GEMM,BFU-E of group C all were a little higher than that of group D.It is concluded that human placental extract in different period without any exogenous cell factors all can support the proliferation of hematopoietic stem cells,this ability is getting stronger with time increasing.The colony formation ability of the amplified cells shows weakened after the first increase,this colony formation ability of the amplified cells in group C is strongest,slightly stronger than that of group D.

关 键 词：人胎盘 胎盘绒毛组织 脐血CD34+细胞 体外培养 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]