干扰素γ对人脐带间充质基质细胞黏附分子表达的影响  

Effects of Interferon-γ on Expression of Adhesion Molecules in Human Umbilical Cord Mesenchymal Stromal Cells

作　　者：乔瑞云[1,2] 白海[1] 王存邦[1] 欧剑峰[1] 张海英[1] 赵强[1] 

机构地区：[1]兰州军区兰州总医院血液科 全军血液病中心,甘肃兰州730050 [2]兰州大学第二临床医学院血液科,甘肃兰州730050

出　　处：《中国实验血液学杂志》2012年第5期1195-1199,共5页Journal of Experimental Hematology

基　　金：甘肃省科技重大专项(编号1102FKDA005)

摘　　要：本研究观察干扰素γ(IFN-γ)对体外培养人脐带间充质基质细胞(UC-MSC)表面黏附分子表达水平的影响。采用组织块移行法培养人UC-MSC,并进行细胞表面抗原、成骨和成脂鉴定。向第3代UC-MSC加入不同浓度的IFN-γ,干预24 h后收集细胞,应用流式细胞仪检测CD54、CD58、CD62p、CD62L、CD44、CD49d、CD102及CD106黏附分子的表达水平。结果表明,生理状态下,CD106、CD62P、CD62L和CD102阳性表达率极低(均<1%),CD54表达最高(41.58±0.83)%;经IFN-γ干预后,CD102、CD106、CD62L、CD62p阳性表达率略有升高,但总体变化不明显(均<5%);CD54、CD58阳性表达率与IFN-γ呈浓度依赖性,最高达(59.66±1.36)%,(43.96±0.62)%;CD49d的阳性表达率在100 U/ml时达到峰值(51.33±0.74)%,CD44在浓度为1 000 U/ml时阳性表达率最高(73.22±1.93)%。结论:IFN-γ可显著提高UC-MSC表面CD54、CD58、CD44、CD49d的阳性表达率,但对CD102、CD106、CD62P和CD62L作用不明显。This study was purposed to investigate the effects of interferon（IFN）-γ on expression of adhesion molecules in mesenchymal stromal cells derived from human umbilical cord tissue（UC-MSC）.The UC-MSC were isolated from human umbilical cord by tissue culture.The expressions of specific markers on UC-MSC were detected by flow cytometry in the physiological condition.The adipogenic and osteogenic induction of UC-MSC was detected by alizarin and Oil red O staining.UC-MSC were exposed to IFN-γ（100,1 000,10 000 U/ml） for 24 h,the expressions of CD54,CD58,CD44,CD49d,CD62p,CD62L,CD102 and CD106 on cell surface were detected using flow cytometry.The results showed that in physiological condition,UC-MSC extremely low expressed CD102,CD106,CD62P,CD62L,while the expression of CD54 was relatively high（41.58±0.83）%.When stimulated by IFN-γ,the expression of CD102,CD106,CD62P,CD62L increased slightly,but still low（5%）,while CD54 and CD58 upregulated concentration-dependently up to（59.66±1.36）% and（43.96±0.62）% respectively.The expression of CD49d upregulated to（51.33±0.74）% when UC-MSC exposed to IFN-γ 100 U/ml.CD44 increased to（73.22±1.93）% when UC-MSC exposed to IFN-γ 1 000 U/ml.It is concluded that IFN-γ can elevate significantly the expression of CD54,CD49d,CD44 and CD58,but has no significant effect on CD102,CD106,CD62P and CD62L expression on the surface of UC-MSC.

关 键 词：脐带间充质基质细胞 黏附分子 干扰素Γ 

分 类 号：R457.7[医药卫生—治疗学] Q813[医药卫生—临床医学]