模拟微重力环境下虫草多糖促进淋巴细胞增殖和CD分子表达的实验研究  

Cordyceps Sinensis Enhances Lymphocyte Proliferation and CD Markers Expression in Simulated Microgravity Environment

作　　者：郝彤[1] 李俊杰[1] 杜芝燕[1] 段翠密[1] 王滟濛[1] 王常勇[1] 宋锦苹[2] 王林杰[2] 李莹辉[2] 王妍[1] 

机构地区：[1]军事医学科学院基础医学研究所前沿交叉学科研究室,北京100850 [2]中国航天员科研训练中心 航天医学基础与应用国家重点实验室,北京100094

出　　处：《中国实验血液学杂志》2012年第5期1212-1215,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金青年科学基金项目(编号31200733);载人航天领域第一批预先研究项目资助(编号Grant No.010201)

摘　　要：本研究旨在探索旋转式细胞培养系统(RCCS)模拟微重力环境下虫草多糖促进淋巴细胞增殖和表面CD分子表达的作用。体外分离培养小鼠脾淋巴细胞,在RCCS模拟微重力环境中分别添加6.25、12.5、25和50μg/ml的虫草多糖溶液培养,在24、48和72 h检测虫草多糖对淋巴细胞增殖及表面CD分子表达的作用。结果表明,模拟微重力环境抑制了淋巴细胞的增殖能力,25和50μg/ml虫草多糖对淋巴细胞的增殖和CD4、CD8的表达均具有促进作用,但50μg/ml虫草多糖促进淋巴细胞增殖的作用随时间延长变为抑制作用。结论:适宜浓度范围内的虫草多糖具有对抗模拟微重力环境下淋巴细胞增殖能力下降和表面CD分子表达减少的作用,为筛选对抗失重效应、促进免疫力的药物提供理论依据和实验基础。This study was aimed to explore the effect of cordyceps sinensis enhancing lymphocyte proliferation and surface CD marker expression in simulated microgravity environment.The splenic lymphocytes were separated from mice and cultured in the rotary cell culture system simulated microgravity environment.The cells were treated with different concentration of cordyceps sinensis solution（0,6.25,12.5,25 and 50 μg/ml） for 24,48 and 72 h respectively,then the cells were harvested,and analyzed for cell proliferation and the expression of cell surface markers（CD4 and CD8）.The results showed that under simulated microgravity environment,the lymphocyte proliferation was inhibited.When the concentration of cordyceps sinensis was 25 or 50 μg/ml,the lymphocyte proliferation,CD4 and CD8 expressions all increased,but 50 μg/ml cordyceps sinensis could inhibit the proliferation ability with the time prolonging.It is concluded that the suitable concentration of cordyceps sinensis displayed the ability to enhance the lymphocyte proliferation and CD marker expression in simulated microgravity environment.These results may be valuable for screening drugs which can be potentially against immunosuppression under simulated microgravity.

关 键 词：虫草多糖 淋巴细胞 模拟微重力 提高免疫力 

分 类 号：R852.22[医药卫生—航空、航天与航海医学] R856.5[医药卫生—临床医学]