中国南京地区汉族人群血小板HPA-1-18遗传多态性研究  

Genetic Polymorphism of Human Platelet Antigens 1-18 in Chinese Nanjing Han Population

作　　者：薛敏[1] 刘衍春[1] 魏鹏[1] 

机构地区：[1]江苏省血液中心,江苏南京210042

出　　处：《中国实验血液学杂志》2012年第5期1235-1239,共5页Journal of Experimental Hematology

摘　　要：本研究旨在调查南京地区无关健康人群人类血小板抗原1-18(HPA-1-18)等位基因频率,为输血患者寻找相容性血小板提供可靠依据。使用PCR-SSP方法对300例南京地区非血缘健康志愿者血液样品进行人血小板抗原1-18(HPA-1-18)等位基因分型。结果表明,南京地区300例非血缘人群HPA等位基因频率分别为:HPA-2a 0.9183和-2b 0.0817;HPA-3a 0.6100和-3b 0.3900;HPA-5a 0.9733和-5b 0.0267;HPA-6a 0.9883和-6b 0.0117;HPA-15a 0.5250和-15b 0.4750。HPA-1a,-4a,-7a-14a和HPA-16a-18a均是纯合子。结论:HPA抗原等位基因频率存在种族和地域差异。南京地区HPA抗原等位基因频率显示具有自身特点。HPA-3和HPA-15是最常见的杂合子,因此必须关注HPA在血小板临床输血中的作用。The purpose of this study was to investigate the allele frequencies of the human platelet alloantigens 1-18 system（HPA-1-18） in Chinese Nanjing unrelated and healthy Han population,so as to provide the credible basis to screen compatible platelets for transfusing patients.The genotypes of 18 HPA systems were determined by polymerase chain reaction using sequence-specific primer（PCR-SSP） for 300 samples.The results showed that the gene frequencies obtained from 300 Nanjing unrelated population were 0.9183 and 0.0817 for HPA-2a and-2b,0.6100 and 0.3900 for HPA-3a and-3b,0.9733 and 0.0267 for HPA-5a and-5b,0.9883 and 0.0117 for HPA-6a and-6b,0.5250 and 0.4750 for HPA-15a and-15b.All the tested individuals were homozygotes for HPA-1a,-4a,-7a-14a and HPA-16a-18a.There was a good fit to Hardy-Weinberg equilibrium in each group.It is concluded that this study has confirmed the ethnic and regional difference of HPA,and HPA in Nanjing Han population has its own characteristics.The highest heterozygotes are HPA-3 and HPA-15,thus more atlention to HPA effects on clinical platelet matched transfusion should be paid.

关 键 词：人血小板抗原系统 基因分型 基因频率 中国南京地区汉族 

分 类 号：R331.143[医药卫生—人体生理学]