LPS促进磨屑诱导分泌溶骨性因子对人工关节松动的影响  

Effect of LPS on osteolysis induced by wear particles in vitro

作　　者：朱自强[1] 段钢[1] 汪明星[1] 王建强[1] 周雷[1] 

机构地区：[1]徐州医学院第二附属医院,江苏221006

出　　处：《齐齐哈尔医学院学报》2012年第19期2632-2633,共2页Journal of Qiqihar Medical University

摘　　要：目的探讨脂多糖(LPS)促进磨屑诱导分泌溶骨性因子M-CSF、IL-1在人工关节松动中的作用。方法采集30例人体外周血,分离单核细胞(MOs),分组培养,A组(对照组):仅MOs;B组:MOs+超高分子聚乙烯微粒(UHMWPE,未去除LPS);C组:MOs+LPS;D组:MOs+UHMWPE(去除LPS);E组:MOs+UHMWPE(去除LPS)+LPS。培养48h后,放免法测定上清中巨噬细胞集落刺激因子(M-CSF)、白介素1(IL-1)的含量。结果 E组M-CSF、IL-1含量明显高于A、B、C、D组(P<0.05);B组M-CSF、IL-2较D组增高(P<0.05)。结论 LPS具有促进UHMWPE刺激M-CSF、IL-1分泌的作用,增强了UHMWPE的溶骨效应。Objective To explore the possibility of Lipopolysaccharide（LPS） contribute to aseptic loosening of orthopedic implants. Methods Peripheral blood samples were obtained from healthy human donors.Monocytes（MOs） were isolated and assigned to 5 groups.Group A was treated with MOs alone,group B with MOs ＋ ultrahigh molecular weight polylethylene（UHMWPE） debris,group C with MOs ＋ LPS（100ng/ ml）,group D with MOs ＋ UHMWPE（LPS removal）,group E with MOs ＋ U HMWPE（LPS removal） ＋ LPS（100ng/ ml）.The cells were co-cultured for 48 hours and the mixtures were tested for osteolytic factors includingM-CSF IL-1. Results The levels of these factors were much higher in group E than in the other 4 groups.There was significant difference between group B and group D（P0.05）. Conclusions LPS can stimulate MOs to secrete osteoclast-activating cytokines,LPS and UHMWPE cooperative interact to enhance the biological effects of aseptic loosening.

关 键 词：脂多糖 假体失败 骨溶解 M-CSF IL-1 

分 类 号：R687.4[医药卫生—骨科学]