骨计量学方法在骨质疏松模型中的定量观察  

Histomorphometry in the quantitative analysis of osteoporosis in rat models

作　　者：于顺禄[1] 魏学磊[1] 熊光宜[1] 徐丽娜[1] 王军[2] 

机构地区：[1]天津市天津医院骨研所,天津300211 [2]第四军医大学全军骨科研究所,西安710032

出　　处：《中国体视学与图像分析》2012年第3期207-214,共8页Chinese Journal of Stereology and Image Analysis

摘　　要：目的观察骨结构与骨质疏松关系即骨构筑学的定量测量"骨质量"的价值,探讨骨组织形态计量学与μCT三维重建计量方法的价值意义。方法采用30只SD雌性大鼠,分成假手术组、切除卵巢去势组和去势后服用阿伦膦酸钠组,每组10只。在服药100 d后处死,进行全股骨骨密度测量及股骨下端不脱钙切片的骨计量学观察。三维重建采用美国GE公司eXplore Locus SP型μCT,用Micview V2.1.2重建软件选取股骨下端骨骺板下2 mm3兴趣窗进行结构计量测量。结果去势后骨体积密度显著性下降,由假手术组的(53.00±3.20)%降至(19.16±2.97)%(P<0.001);经二膦酸盐治疗后得到显著性改善(升至(40.40±3.07)%,与去势组比较P<0.001)。同时假手术组与去势组和去势组与二膦酸盐组之间,骨计量学指标中四环素标记率,类骨质的表面密度、平均类骨质表面厚度及动态指标,骨矿化沉积率与骨矿化延迟时间等均有显著性差异(P<0.05)。μCT结果显示骨小梁体积比与骨表面积体积比显示结果同骨计量学结果相似,数值均同比例减少。另外在三维结构计量上,除骨小梁厚度外,骨小梁数目、骨小梁间隙等均出现显著性变化。结论骨组织形态计量学与显微CT三维重建定量分析对骨结构与骨质量指标均能进行定性、定量的客观评价;骨计量学方法能从静态和动态两个方面反映骨新陈代谢过程,可以精确地反映出骨重建过程的变化规律和特点。Objective In order to clearly clarify the relationships between bone architecture and osteopo- rosis, namely, quantitatively evaluate the values of bone quality, a comparative analysis of samples of os- teoporosis models was performed by bone tissue histomorphometry and 3-D reconstruction of micro-CT ima- ges, and to explore the values of those two techniques. Methods Thirty healthy adult female Spraque- Dawley rats were included in this study. The rats were randomly divided into sham group, ovariectomized group and ovariectomy ＋ alendronate sodium group （n = 10, each）. The rats were sacrificed at 100 days after drug administration. The bone mass density （BMD） of the whole femur was measured and his- tological sections of the undecalcified bone tissue of the distal end of femur were prepared for morpho- metric observation. A region of 2 mm3 bone tissue beneath the epiphyseal plate of the distal end of femur was used for morphometric measurement to make 3-D reconstruction of micro-CT images （ explore Locus SP, GE, USA） using software Micview V2.1.2. Results The bone mass density in the ovariecto- mized gourp was （ 19.16 ±2.97） % , significantly decreased than that （53.00 ±3.20） % in the sham group [ （53.00 ± 3.20） % ] , （ P 〈 0.001 ）, and was （40.40 ±3.07 ） after the alendronate sodium treatment, significantly improved than that of the ovariectomized gourp （P 〈 0. 001 ）. There were signif- icant differences in the values of tetracycline labeling rate, osteoid surface density, average osteoid for- mation surface thickness, mineralization apposition rate and bone mineralization delay time between the sham group and ovariectomied groups, and between the ovariectomized and alendronate sodium treat- ment groups（P 〈 0.05）. The 3-D reconstruction of miero-CT observation showed that the bone trabec- ular volume ratio （BV/TV） and bone surface/bone volume ratio, BS/BV） were similar to those ob- tained by histomorphometry, except that the absolute values were smaller.

关 键 词：骨组织形态计量学 骨质疏松模型 显微CT骨结构 骨质量 大鼠 

分 类 号：R580[医药卫生—内分泌]