ACF/PLGA骨组织工程复合支架的制备及其性能  

Preparation and Properties of ACF/PLGA Scaffolds for Bone Tissue Engineering

作　　者：韩浩[1] 李利伟[1] 秦文贞[1] 全海宇[1] 吴琪琳[1,2] 

机构地区：[1]东华大学材料科学与工程学院,上海201620 [2]东华大学纤维改性国家重点实验室,上海201620

出　　处：《材料科学与工程学报》2012年第5期719-723,共5页Journal of Materials Science and Engineering

基　　金：国家自然科学基金资助项目(60975059)

摘　　要：本文利用溶剂灌制/粒子沥滤的方法将具有较强吸附性能的活性碳纤维（activated carbon fiber,ACF）掺杂于聚乳酸-羟基乙酸共聚物（poly（lactic-co-glycolic acid）,PLGA）制备了一种新型ACF/PLGA骨组织工程复合支架。论文对比研究了纯PLGA支架以及两种ACF/PLGA支架（ACF含量为2.75%,8.26%）的结构和性能。SEM研究发现三者都具有较高的孔隙度,分别为73.5340%、75.1214%和79.8216%,且孔隙度随着ACF含量的增加逐渐增大;压汞法测得三者的孔径分布基本在50~250μm之间;研究其亲水性发现,其表面接触角随ACF含量增加逐渐减小,吸水率则逐渐增大。进一步研究发现在三种支架上种植小鼠成纤维细胞（L929）,一天后细胞都较好粘附在支架上;ACF含量为8.26%的复合支架移植到小白鼠皮下组织,一月后HE切片显示支架周围组织的免疫排斥反应较小。掺杂ACF的PLGA复合支架除了具有良好的细胞粘附效果和组织相容性,相对于纯PLGA支架,还具有良好的孔径分布和亲水性,具有潜在的应用价值。Novel ACF/PLGA bone tissue engineering composite scaffolds were prepared by solvent casting/particulate leaching method,within which activated carbon fibers（ACF） with high adsorbility were incorporated into poly（lactic-co-glycolic acid）（PLGA）.Structure and properties of the scaffolds were studied by comparing monotonic PLGA and two ACF/PLGA scaffolds（mass fractions of ACF are 2.75% and 8.26%,respectively）.SEM showed that the three scaffolds were of high porosity,approximately increasing with increasing content of ACF from 73.5340%,75.1214% to 79.8216%.The mercury injection method revealed that pore size distributions of all scaffolds were between 50-250μm.The hydrophilicity investigation implied that the contact angle decreased and the water absorption increased when the content of ACF increased.SEM observed that L929 fibroblast adhered well on the scaffolds after mouse fibroblast（L929） was cultured on the scaffolds for 1 day.When the scaffolds have implanted to subcutaneous tissue of mice for a month,HE sections showed that tissue around scaffolds were immunoreactive at low level.Compared with PLGA,ACF/PLGA bone tissue engineering scaffolds possessed better pore size distribution,hydrophilicity.good cell adhesion and histocompatibility,indicating a potential application value.

关 键 词：骨组织工程 支架材料 PLGA ACF 

分 类 号：R318.08[医药卫生—生物医学工程]